Mouse splenocytes were stained with Rat Anti-Mouse MARCO APC-conjugated Monoclonal Antibody (Catalog # FAB2956A, filled histogram) or isotype control antibody (Catalog # IC005A, open histogram). View our protocol for Staining Membrane-associated Proteins.

Detection of MARCO in J774 Mouse Cell Line by Flow Cytometry.

J774 mouse monocyte-macrophage cell line, resting (open histogram), or treated with 500 ng/ml LPS for 3 days (filled histogram) were stained with Rat Anti-Mouse MARCO APC-conjugated Monoclonal Antibody (Catalog # FAB2956A). View our protocol for Staining Membrane-associated Proteins.

Applications for Mouse MARCO APC-conjugated Antibody

Application

Recommended Usage

Flow Cytometry

10 µL/10⁶ cells
Sample: Mouse splenocytes or J774 mouse cell line treated with LPS

Reviewed Applications

Read 1 review rated 4 using FAB2956A in the following applications:

Flow Cytometry (1 Review)

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Formulation

Supplied in a saline solution containing BSA and Sodium Azide.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze.

12 months from date of receipt, 2 to 8 °C as supplied.

Background: MARCO

Mouse MARCO is a type II transmembrane glycoprotein belonging to the class A scavenger receptor family. It is constitutively expressed in subsets of macrophages found in the marginal zone of the spleen, the peritoneum, and the medullary cord of lymph nodes. The extracellular domains of MARCO form a 220 kDa disulfide-linked homotrimer on the cell surface. MARCO binds both gram positive and gram negative bacteria, as well as oxidized low-density lipoprotein. The amino acid sequence of mouse MARCO extracellular domain is 69% identical to that of human MARCO.

Long Name

Macrophage Receptor with Collagenous Structure/Scavenger Receptor, Class A, Member 2

Alternate Names

SCARA2

Entrez Gene IDs

8685 (Human); 17167 (Mouse)

Gene Symbol

MARCO

UniProt

Q60754

Additional MARCO Products

Product Documents for Mouse MARCO APC-conjugated Antibody

Product Datasheets

Download Product Datasheets

COA

SDS

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Product Specific Notices for Mouse MARCO APC-conjugated Antibody

For research use only

Citations
Reviews

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Flow Cytometry

Mouse MARCO APC-conjugated Antibody

R&D Systems, part of Bio-Techne | Catalog # FAB2956A

Key Product Details

Validated by

Species Reactivity

Validated:

Cited:

Applications

Validated:

Cited:

Label

Antibody Source

View all MARCO Antibodies »

Product Specifications

Immunogen

Specificity

Clonality

Host

Isotype

Scientific Data Images for Mouse MARCO APC-conjugated Antibody

Detection of MARCO in Mouse Splenocytes by Flow Cytometry.