Mouse Mast Cell Protease-11/Prss34 Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB2857
Key Product Details
Species Reactivity
Mouse
Applications
Immunoprecipitation, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Rat IgG1 Clone # 451421
Product Specifications
Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse Mast Cell Protease‑11/Prss34
Met20-Ser318
Accession # Q80UR4
Met20-Ser318
Accession # Q80UR4
Specificity
Detects mouse Mast Cell Protease‑11/Prss34 in direct ELISAs and Western blots. In direct ELISAs and Western blots, no cross-reactivity with recombinant mouse (rm) MCP-1, rmMCP-6, or rmMCP-7 is observed.
Clonality
Monoclonal
Host
Rat
Isotype
IgG1
Applications for Mouse Mast Cell Protease-11/Prss34 Antibody
Application
Recommended Usage
Immunoprecipitation
25 µg/mL
Sample: Conditioned cell culture medium spiked with Recombinant Mouse Mast Cell Protease-11/Prss34 (Catalog # 2857-SE), see our available Western blot detection antibodies
Sample: Conditioned cell culture medium spiked with Recombinant Mouse Mast Cell Protease-11/Prss34 (Catalog # 2857-SE), see our available Western blot detection antibodies
Western Blot
1 µg/mL
Sample: Recombinant Mouse Mast Cell Protease-11/Prss34 (Catalog # 2857-SE)
Sample: Recombinant Mouse Mast Cell Protease-11/Prss34 (Catalog # 2857-SE)
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Mast Cell Protease-11/Prss34
Mast Cell Protease-11 (MCP-11) is encoded by Prss34, one of 13 genes on mouse chromosome 17A3.3 that correspond to functional trypsin-like serine proteases (1). The deduced amino acid sequence of mouse MCP-11 consists of 318 residues with a signal peptide (residues 1 to 19), a pro region (residue 20 to 34), and a catalytic domain (35 to 318). The mRNA is preferentially expressed in spleen and bone marrow. The amino acid sequence of mouse MCP-11 shares 84% and 60% identity with that of rat and dog, respectively. The human gene corresponding to Prss34 contains a mutation that leads to a premature translation termination codon, thus encoding a protein that is unlikely to be enzymatically active.
References
- Wong, G.W. et al. (2004) J. Biol. Chem. 279:2438.
Alternate Names
Mast Cell Protease11, MCP-11, MCPT11, Prss34
Entrez Gene IDs
328780 (Mouse)
Gene Symbol
PRSS34
UniProt
Additional Mast Cell Protease-11/Prss34 Products
Product Documents for Mouse Mast Cell Protease-11/Prss34 Antibody
Product Specific Notices for Mouse Mast Cell Protease-11/Prss34 Antibody
For research use only
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