Mouse MGMT Antibody
R&D Systems, part of Bio-Techne | Catalog # AF3299
Key Product Details
Species Reactivity
Mouse
Applications
Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Goat IgG
Product Specifications
Immunogen
E. coli-derived recombinant mouse MGMT
Met1-Asn211
Accession # P26187
Met1-Asn211
Accession # P26187
Specificity
Detects mouse MGMT in Western blots.
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Scientific Data Images for Mouse MGMT Antibody
Detection of Mouse MGMT by Western Blot.
Western blot shows lysates of Balb/3T3 mouse embryonic fibroblast cell line and CH-1 mouse B cell lymphoma cell line. PVDF membrane was probed with 1 µg/mL of Mouse MGMT Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3299) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for MGMT at approximately 25 kDa (as indicated). This experiment was conducted using Immunoblot Buffer Group 1.Applications for Mouse MGMT Antibody
Application
Recommended Usage
Western Blot
1 µg/mL
Sample: Balb/3T3 mouse embryonic fibroblast cell line and CH-1 mouse B cell lymphoma cell line
Sample: Balb/3T3 mouse embryonic fibroblast cell line and CH-1 mouse B cell lymphoma cell line
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: MGMT
The O-6-methylguanine-DNA-methyl transferase (MGMT) is involved in the direct repair of DNA alkylated at the O6 position of guanine. MGMT transfers the alkyl group to its own cysteine thereby inactivating the MGMT molecule. Therefore, the number of MGMT molecules correlates directly with the cell’s ability to withstand DNA alkylation.
Long Name
O-6 Methylguanine-DNA Methyltransferase
Alternate Names
6-O-methylguanine-DNA methyltransferase, EC 2.1.1.63, methylated-DNA--protein-cysteine methyltransferase, methylguanine-DNA methyltransferase, O-6-methylguanine-DNA methyltransferase, O6-methylguanine-DNA methyltransferase, O-6-methylguanine-DNA-alkyltransferase
Gene Symbol
MGMT
UniProt
Additional MGMT Products
Product Documents for Mouse MGMT Antibody
Product Specific Notices for Mouse MGMT Antibody
For research use only
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