Mouse Mimecan Antibody
R&D Systems, part of Bio-Techne | Catalog # AF2949
Key Product Details
Species Reactivity
Validated:
Mouse
Cited:
Mouse, Bovine
Applications
Validated:
Western Blot
Cited:
Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Goat IgG
Product Specifications
Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse Mimecan
Ala20-Phe298
Accession # Q62000
Ala20-Phe298
Accession # Q62000
Specificity
Detects mouse Mimecan in direct ELISAs and Western blots. In direct ELISAs, approximately 10% cross-reactivity with recombinant human Mimecan is observed.
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Applications for Mouse Mimecan Antibody
Application
Recommended Usage
Western Blot
0.1 µg/mL
Sample: Recombinant Mouse Mimecan (Catalog # 2949-MC)
Sample: Recombinant Mouse Mimecan (Catalog # 2949-MC)
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Mimecan
Mimecan, also known as KSPG25 (25 kDa Keratan Sulfate Proteoglycan) and Osteoglycin, is an extracellular matrix protein belonging to the class III subfamily of the small leucine rich proteoglycan (SLRP) family. SLRPs are characterized by leucine rich repeats in the central domain, which is flanked by N- and C-terminal cysteine‑rich domains. The core proteins contain N-linked oligosaccharides as well as glycosaminoglycan side chains. Mimecan is synthesized as a preproprotein that is processed to generate proteins of different sizes including Osteoglycin (containing 93 most C‑terminal amino acid residues) and Mimecan (containing 223 most C‑terminal residues). Mouse Mimecan shares approximately 94% and 85% amino acid sequence identity with rat and human Mimecan, respectively.
Alternate Names
KSPG25, OGN, OIF, SLRR3A
Gene Symbol
OGN
UniProt
Additional Mimecan Products
Product Documents for Mouse Mimecan Antibody
Product Specific Notices for Mouse Mimecan Antibody
For research use only
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