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Mouse MOG Antibody

R&D Systems, part of Bio-Techne | Catalog # AF2439

R&D Systems, part of Bio-Techne
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AF2439
AF2439-SP

Key Product Details

Species Reactivity

Validated:

Mouse

Cited:

Mouse

Applications

Validated:

Immunohistochemistry, Western Blot

Cited:

Immunocytochemistry, Immunohistochemistry, Immunohistochemistry-Frozen

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant mouse MOG
Gly29-Gly153
Accession # Q61885

Specificity

Detects mouse MOG in direct ELISAs and Western blots. In direct ELISAs, less than 40% cross-reactivity with recombinant human MOG is observed.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Mouse MOG Antibody

MOG antibody in Mouse Brain by Immunohistochemistry (IHC-Fr).

MOG in Mouse Brain.

MOG was detected in perfusion fixed frozen sections of mouse brain (cortex) using 15 µg/mL Goat Anti-Mouse MOG Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2439) overnight at 4 °C. Tissue was stained with the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.

Applications for Mouse MOG Antibody

Application
Recommended Usage

Immunohistochemistry

5-15 µg/mL
Sample: Perfusion fixed frozen sections of mouse brain (cortex)

Western Blot

0.1 µg/mL
Sample: Recombinant Mouse MOG
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

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Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: MOG

Mouse MOG is an integral membrane protein belonging to the immunoglobulin superfamily. It is found exclusively on myelinating oligodendrocytes in the central nervous system. The amino-terminal extracellular domain of mouse MOG shares 97% and 92% amino acid sequence identity with the corresponding regions of rat and human MOG, respectively.

Long Name

Myelin Oligodendrocyte Glycoprotein

Alternate Names

MGC26137, MOG Ig-AluB, MOGIG2, myelin oligodendrocyte glycoprotein, myelin-oligodendrocyte glycoprotein

Entrez Gene IDs

4340 (Human); 17441 (Mouse)

Gene Symbol

MOG

UniProt

Additional MOG Products

Product Documents for Mouse MOG Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Mouse MOG Antibody

For research use only

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