Detects mouse Neuropilin-1 in direct ELISAs. In direct ELISAs, less than 5% cross-reactivity with recombinant rat (rr) Neuropilin-1 is observed and no cross-reactivity with recombinant human (rh) Neuropilin-1, rhNeuropilin-2, or rrNeuropilin-2 is observed.

Clonality

Monoclonal

Host

Rat

Isotype

IgG_2A

Scientific Data Images for Mouse Neuropilin-1 PE-conjugated Antibody

Detection of Neuropilin‑1 in bEnd.3 Mouse Cell Line by Flow Cytometry.

bEnd.3 mouse endothelioma cell line was stained with Rat Anti-Mouse Neuropilin-1 PE-conjugated Monoclonal Antibody (Catalog # FAB5994P, filled histogram) or isotype control antibody (Catalog # IC006P, open histogram). View our protocol for Staining Membrane-associated Proteins.

Applications for Mouse Neuropilin-1 PE-conjugated Antibody

Application

Recommended Usage

Flow Cytometry

10 µL/10⁶ cells
Sample: bEnd.3 mouse endothelioma cell line

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Formulation

Supplied in a saline solution containing BSA and Sodium Azide.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store the unopened product at 2 - 8° C. Do not use past expiration date. Protect from light.

Background: Neuropilin-1

Neuropilin-1 (Nrp-1, previously neuropilin; also CD304) is a 130 - 140 kDa type I transmembrane (TM) glycoprotein that regulates axon guidance and angiogenesis. The full-length 923 amino acid (aa) mouse Nrp-1 contains a 623 aa extracellular domain (ECD) that shares 98% aa identity with rat and 93% aa identity with human, equine, bovine and canine Nrp-1. The ECD contains two N-terminal CUB domains (termed a1a2), two domains with homology to coagulation factors V and VIII (b1b2) and a MAM (meprin) domain (c). At least one splice variant that diverges at aa 587 and lacks the TM domain has been sequenced. This form is potentially a soluble antagonist, based on results from human Nrp-1 splice variants. The sema domains of Class III secreted semaphorins such as Sema3A bind Nrp-1 a1a2. Heparin, the heparin-binding forms of VEGF (VEGF₁₆₅, VEGF-B and VEGF-E), PlGF (PlGF2), plus the C-terminus of Sema3 bind the b1b2 region of Nrp-1. Nrp-1 and Nrp-2 share 48% aa identity within the ECD and can form homo- and hetero-oligomers via interaction of their MAM domains. Neuropilins show partially overlapping expression in neuronal and endothelial cells during development. Both neuropilins act as co-receptors with plexins, including plexins A1, A4, B1, and D1, to bind class III semaphorins, such as Sema 3A, 3C, 3D, 3E, and 3F, that often mediate axon repulsion. Both Nrp-1 and Nrp-2 serve as co-receptors with VEGF R2 (also called KDR or Flk-1) for VEGF₁₆₅ binding. Sema3A signaling can be blocked by VEGF₁₆₅, which has higher affinity for Npn-1. Nrp-1 is preferentially expressed in developing or remodeling arteries. Nrp-1 is also expressed on dendritic cells and mediates DC-induced T cell proliferation.

Alternate Names

BDCA-4, CD304, Neuropilin1, NRP1

Entrez Gene IDs

8829 (Human); 18186 (Mouse); 246331 (Rat); 100286859 (Porcine)

Gene Symbol

NRP1

UniProt

P97333 (Mouse)

Additional Neuropilin-1 Products

Product Documents for Mouse Neuropilin-1 PE-conjugated Antibody

Product Datasheets

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COA

SDS

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Product Specific Notices for Mouse Neuropilin-1 PE-conjugated Antibody

For research use only

Citations
Reviews

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Flow Cytometry

Mouse Neuropilin-1 PE-conjugated Antibody

R&D Systems, part of Bio-Techne | Catalog # FAB5994P

Key Product Details

Species Reactivity

Validated:

Cited:

Applications