Mouse Neuropoietin/NP Antibody
R&D Systems, part of Bio-Techne | Catalog # AF2709
Key Product Details
Species Reactivity
Mouse
Applications
Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Goat IgG
Product Specifications
Immunogen
E. coli-derived recombinant mouse Neuropoietin/NP
Ala23-Ala204
Accession # P83714
Ala23-Ala204
Accession # P83714
Specificity
Detects mouse Neuropoietin/NP in direct ELISAs and Western blots.
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Applications for Mouse Neuropoietin/NP Antibody
Application
Recommended Usage
Western Blot
0.1 µg/mL
Sample: Recombinant Mouse Neuropoietin/NP (Catalog # 2709-NP)
Sample: Recombinant Mouse Neuropoietin/NP (Catalog # 2709-NP)
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Neuropoietin/NP
Neuropoietin (NP; also known as cardiotrophin-2) is a 22 kDa member of the IL-6 family of cytokines. Considered to be the product of a gene duplication event involving cardiotrophin-1 (CT-1), it helps to define a subfamily within the IL-6 family that includes CT-1, CLC and CTNF. Mouse neuropoietin is synthesized as a 204 amino acid (aa) precursor that contains a 22 aa signal sequence and a 192 aa mature segment. The secreted molecule is characterized by the presence of four alpha‑helices, typical of hematopoietic superfamily molecules. Mature mouse neuropoietin shares 88%, 90% and 95% aa identity to chimpanzee, canine and rat neuropoietin, respectively. The human gene is suggested to have evolved towards a pseudogene, a point of interest in that neuropoietin is reported to signal through the CNTF complex (i.e., gp130, CNTF R alpha and LIF R). NP will mediate motor neuron survival, and appears to be selectively expressed in the embryo by tissues involved with nervous system development (1).
References
- Derouet, D. et al. (2004) Proc. Natl. Acad. Sci. USA 101:4827.
Alternate Names
Cardiotrophin-2, CTF2P, NP
Gene Symbol
LOC647088
UniProt
Additional Neuropoietin/NP Products
Product Documents for Mouse Neuropoietin/NP Antibody
Product Specific Notices for Mouse Neuropoietin/NP Antibody
For research use only
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