Mouse Noggin Antibody
R&D Systems, part of Bio-Techne | Catalog # AF719
Key Product Details
Species Reactivity
Validated:
Mouse
Cited:
Human, Mouse, Rat, Avian - Chicken, Rabbit
Applications
Validated:
Immunocytochemistry, Immunohistochemistry, Western Blot
Cited:
Functional Assay, Immunocytochemistry, Immunohistochemistry, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin
Label
Unconjugated
Antibody Source
Polyclonal Goat IgG
Product Specifications
Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse Noggin
Leu20-Cys232
Accession # P97466
Leu20-Cys232
Accession # P97466
Specificity
Detects mouse Noggin in direct ELISAs and Western blots. In direct ELISAs, approximately 30% cross-reactivity with recombinant human Noggin is observed.
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Scientific Data Images for Mouse Noggin Antibody
Noggin in Embryonic Mouse Cardiac Tissue.
Noggin was detected in immersion fixed frozen sections of embryonic mouse cardiac tissue (11 d.p.c.) using 15 µg/mL Goat Anti-Mouse Noggin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF719) overnight at 4 °C. Tissue was stained with the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.
Noggin in PC‑3 Human Cell Line.
Noggin was detected in immersion fixed PC-3 human prostate cancer cell line using Goat Anti-Mouse Noggin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF719) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red, upper panel; Catalog # NL001) and counterstained with DAPI (blue, lower panel). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.Applications for Mouse Noggin Antibody
Application
Recommended Usage
Immunocytochemistry
5-15 µg/mL
Sample: Immersion fixed PC‑3 human prostate cancer cell line
Sample: Immersion fixed PC‑3 human prostate cancer cell line
Immunohistochemistry
5-15 µg/mL
Sample: Immersion fixed frozen sections of embryonic mouse cardiac tissue (11 d.p.c.)
Sample: Immersion fixed frozen sections of embryonic mouse cardiac tissue (11 d.p.c.)
Western Blot
0.1 µg/mL
Sample: Recombinant Mouse Noggin (Catalog # 1967-NG)
Sample: Recombinant Mouse Noggin (Catalog # 1967-NG)
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Reviewed Applications
Read 5 reviews rated 4.8 using AF719 in the following applications:
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Noggin
Noggin was originally cloned based on its dorsalizing activity in Xenopus embryos. Mammalian Noggins were subsequently identified and cloned from human, mouse and rat cDNA libraries. Mouse Noggin cDNA encodes a 232 amino acid (aa) residue precursor protein with 19 aa residue putative signal peptide that is cleaved to generate the 213 aa residue mature protein which is secreted as a homodimeric glycoprotein. Noggin is a highly conserved molecule. Mature mouse Noggin shares 99% and 83% aa sequence identity with human and Xenopus Noggin, respectively. Noggin has a complex pattern of expression during embryogenesis. In the adult, Noggin is expressed in the central nervous system and in several adult peripheral tissues such as lung, skeletal muscle and skin. Noggin has been shown to be a high-affinity BMP (bone morphogenetic protein) binding protein that antagonizes BMP bioactivities.
References
- Smith, W.C. and R.M. Harland (1992) Cell 70:829.
- Valenzuela, D.M. et al. (1995) J. Neurosci. 15:6077.
- Brunet, L.J. et al. (1998) Science 280:1455.
Alternate Names
NOG, SYM1, SYNS1, SYNS1A
Gene Symbol
NOG
UniProt
Additional Noggin Products
Product Documents for Mouse Noggin Antibody
Product Specific Notices for Mouse Noggin Antibody
For research use only
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