Mouse Plasminogen Kringle 5 Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB742
Key Product Details
Species Reactivity
Validated:
Mouse
Cited:
Mouse
Applications
Validated:
Western Blot
Cited:
Immunocytochemistry
Label
Unconjugated
Antibody Source
Monoclonal Rat IgG1 Clone # 120304
Product Specifications
Immunogen
E. coli-derived recombinant mouse Plasminogen Kringle 5
Cys481-Ala563
Accession # AAA50168
Cys481-Ala563
Accession # AAA50168
Specificity
Detects mouse Plasminogen Kringle 5 in direct ELISAs and Western blots. In direct ELISAs, no cross‑reactivity with recombinant human Plasminogen Kringle 5 is observed.
Clonality
Monoclonal
Host
Rat
Isotype
IgG1
Scientific Data Images for Mouse Plasminogen Kringle 5 Antibody
Detection of Mouse Plasminogen Kringle 5 by Western Blot
Ferulic acid regulated the differential expression of angiogenic and antiangiogenic factors in MN mouse model. At the end of the experiment, kidney samples of different groups were harvested and freshly isolated the glomeruli according to the Materials and Methods. Total protein lysate was extracted from isolated glomeruli and separated in 10% SDS page by electrophoresis, and further blotting by anti-VEGF (a), TSP-1 (b), and K5 (c) antibodies. The bar chart represented the semiquantification level of the expression density (target/actin) (Left Panel) (*P < 0.05; **P < 0.01). Immunofluorescence staining was also undertaken to exam the expression level and location of VEGF, TSP-1, and K5 in NC and N-T, original magnifications, 400x (Right Panel). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/22844329), licensed under a CC-BY license. Not internally tested by R&D Systems.Applications for Mouse Plasminogen Kringle 5 Antibody
Application
Recommended Usage
Western Blot
1 µg/mL
Sample: Recombinant Mouse Plasminogen Kringle 5
Sample: Recombinant Mouse Plasminogen Kringle 5
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Plasminogen Kringle 5
Plasminogen is a coenzyme that has five triple disulfide-linked kringle domains. It is converted to plasmin by plasminogen activators. Internal fragments of plasminogen, including the naturally occurring angiostatin isoforms and Kringle 5, can also be generated by proteolytic cleavage. These internal fragments have been shown to be potent angiogenesis inhibitors (1).
References
- Cao, Y. et al. (1997) J. Biol. Chem. 272:22924.
Alternate Names
HAE4
Gene Symbol
PLG
UniProt
Additional Plasminogen Kringle 5 Products
Product Documents for Mouse Plasminogen Kringle 5 Antibody
Product Specific Notices for Mouse Plasminogen Kringle 5 Antibody
For research use only
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