Mouse PlGF-2 Antibody
R&D Systems, part of Bio-Techne | Catalog # AF465
Key Product Details
Species Reactivity
Validated:
Mouse
Cited:
Mouse, Rat
Applications
Validated:
Western Blot
Cited:
Immunohistochemistry, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Goat IgG
Product Specifications
Immunogen
S. frugiperda insect ovarian cell line Sf 21-derived recombinant mouse PlGF-2 (R&D Systems, Catalog # 465-PL)
Ala24-Pro158
Accession # Q544A5
Ala24-Pro158
Accession # Q544A5
Specificity
Detects mouse PlGF-2 in direct ELISAs and Western blots. In these formats, less than 5% cross‑reactivity with recombinant human PlGF is observed.
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Applications for Mouse PlGF-2 Antibody
Application
Recommended Usage
Western Blot
0.1 µg/mL
Sample: Recombinant Mouse PlGF-2 (Catalog # 465-PL)
Sample: Recombinant Mouse PlGF-2 (Catalog # 465-PL)
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: PlGF-2
Placenta growth factor (PlGF) is a member of the vascular endothelial growth factor (VEGF) family of growth factors. PlGF and VEGF share primary structural as well as limited amino acid sequence homology with the A and B chains of PDGF. All eight cysteine residues involved in intra- and inter-chain disulfides are conserved among these growth factors. As a result of alternative splicing, three PlGF RNAs encoding monomeric human PlGF-1, PlGF-2 and PlGF-3 isoform precursors containing 149, 179 and 219 amino acid residues, respectively, have been described. In normal mouse tissues, only one mouse PlGF mRNA encoding the equivalent of human PlGF-2 has been identified. Mouse PlGF-2 encodes a 158 amino acid residue precursor protein. Mouse PlGF-2 shares 65% amino acid identity with human PlGF-2. The gene for PlGF has been mapped to mouse chromosome 12 and human chromosome 14. PlGF binds with high-affinity to Flt-1, but not to Flk-1/KDR.
References
- DiPalma, T. et al. (1996) Mamm. Genome 7:6.
- Cao, Y. et al. (1997) Biochem. Biophys. Res. Commun. 235:493.
- Ferrara, N. et al. (1997) Endocrin. Rev. 18:4.
Long Name
Placenta Growth Factor 2
Alternate Names
PlGF2
Gene Symbol
PGF
UniProt
Additional PlGF-2 Products
Product Documents for Mouse PlGF-2 Antibody
Product Specific Notices for Mouse PlGF-2 Antibody
For research use only
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