Mouse R-Spondin 3 Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB4120
Key Product Details
Species Reactivity
Validated:
Mouse
Applications
Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Rat IgG2A Clone # 400404
Product Summary for Mouse R-Spondin 3 Antibody
Immunogen
E. coli-derived recombinant mouse R‑Spondin 3
Ser21-Gly209 (Gln108Lys)
Accession # BAC36296
Ser21-Gly209 (Gln108Lys)
Accession # BAC36296
Specificity
Detects mouse R‑Spondin 3 in direct ELISAs and Western blots. In direct ELISAs and Western blots, no cross-reactivity with recombinant mouse (rm) RSPO-1, rmRSPO-4, recombinant human (rh) RSPO-2, or rhRSPO-3 is observed.
Clonality
Monoclonal
Host
Rat
Isotype
IgG2A
Applications for Mouse R-Spondin 3 Antibody
Application
Recommended Usage
Western Blot
1 µg/mL
Sample: Recombinant Mouse R-Spondin 3
Sample: Recombinant Mouse R-Spondin 3
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: R-Spondin 3
RSPO-3 (R-spondin 3; also Cristin-1), is a 256 aa secreted R-Spondin family member which binds LRP-6 and Fzd8 and regulates beta-catenin signaling. R-Spondins contain two adjacent cysteine-rich furin-like domains at their amino-termini, followed by a thrombospondin (TSP-1) motif and a variable length basic C-terminal region. Two RSPO-3 splice isoforms that differ in their C-termini have been reported. Mouse RSPO-3 shares 93% and 97% aa identity with human and rat RSPO-3, respectively, over the sequence used as an immunogen.
Long Name
Roof Plate-specific Spondin 3
Alternate Names
Cristin 1, PWTSR, RSPO3, RSpondin 3, THSD2
Gene Symbol
RSPO3
UniProt
Additional R-Spondin 3 Products
Product Documents for Mouse R-Spondin 3 Antibody
Product Specific Notices for Mouse R-Spondin 3 Antibody
For research use only
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