BaF3 mouse pro-B cell line transfected with Rae-1 beta (Accession # NP_033043) and Mouse myeloma cell line NS0-derived recombinant mouse Rae-1 delta Leu29-Ser227 (Accession # Q9JI58)

Specificity

Detects mouse Rae‑1 alpha/ beta/ delta/ epsilon. When tested in flow cytometry against a panel of transfectants expressing different Rae-1 proteins, this antibody strongly recognizes Rae-1 beta and Rae-1 delta. It shows weaker staining of Rae-1 alpha and Rae-1 epsilon transfectants. It does not recognize Rae-1 gamma.

Clonality

Monoclonal

Host

Rat

Isotype

IgG_2A

Endotoxin Level

<0.10 EU per 1 μg of the antibody by the LAL method.

Applications for Mouse Rae-1 alpha/ beta/ delta/ epsilon Antibody

Application

Recommended Usage

Blockade of Receptor-ligand Interaction

In a functional ELISA, 1-6 ng/mL of this antibody will block 50% of the binding of 300 ng/mL of Biotinylated Recombinant Mouse NKG2D Fc Chimera to immobilized Recombinant Mouse Rae-1 delta Fc Chimera (Catalog # 1134‑RA) coated at 1 µg/mL (100 µL/well). At 100 ng/mL, this antibody will block >90% of the binding.

Blocking of Recombinant Mouse Rae-1 beta is observed at a similar antibody concentration, however, this antibody does not block the binding of Recombinant Mouse NKG2D Fc Chimera to Recombinant Mouse Rae-1 alpha or Rae-1 epsilon in this assay.

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Flow Cytometry

2.5 µg/10⁶ cells
Sample: RAW 264.7 mouse monocyte/macrophage cell line

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Rae-1

Rae-1 alpha, beta, gamma, delta and epsilon (retinoic acid early transcript 1) comprise a family of closely related (88‑95% amino acid identity) GPI-linked cell surface proteins that function as ligands for mouse NKG2D, an activating receptor expressed on NK and T cells. Rae-1 transcripts are expressed in mouse embryos and several tumor cell lines but are absent from most normal adult tissues. Rae-1 protein expression on tumor cell lines has been implicated in in vivo tumor rejection.

Long Name

Retinoic Acid Early Transcript 1

Alternate Names

Rae1

Entrez Gene IDs

8480 (Human); 19368 (Mouse)

Gene Symbol

RAE1

Additional Rae-1 Products

Product Documents for Mouse Rae-1 alpha/ beta/ delta/ epsilon Antibody

Product Datasheets

Download Product Datasheets

COA

SDS

Download SDS

Product Specific Notices for Mouse Rae-1 alpha/ beta/ delta/ epsilon Antibody

For research use only

Citations
Reviews

Calculators
Flow Cytometry

Mouse Rae-1 alpha/ beta/ delta/ epsilon Antibody

R&D Systems, part of Bio-Techne | Catalog # MAB17581

Key Product Details

Species Reactivity

Validated:

Cited:

Applications