Mouse/Rat CCR8 Antibody

R&D Systems, part of Bio-Techne | Catalog # AF5997

R&D Systems, part of Bio-Techne
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AF5997
AF5997-SP
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Key Product Details

Species Reactivity

Mouse, Rat

Applications

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Sheep IgG

View all CCR8 Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant mouse CCR8
Met1-Met33, Asn92-Lys105, Tyr170-Ile200, His262-Leu278
Accession # P56484

Specificity

Detects mouse and rat CCR8 in direct ELISAs and Western blots. In direct ELISAs, less than 2% cross‑reactivity with recombinant mouse (rm) CCR1, rmCCR4, and rmCCR5 is observed.

Clonality

Polyclonal

Host

Sheep

Isotype

IgG

Scientific Data Images for Mouse/Rat CCR8 Antibody

Detection of Mouse and Rat CCR8 antibody by Western Blot.

Detection of Mouse and Rat CCR8 by Western Blot.

Western blot shows lysates of mouse thymus tissue and rat thymus tissue. PVDF Membrane was probed with 0.5 µg/mL of Sheep Anti-Mouse/Rat CCR8 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5997) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for CCR8 at approximately 41-43 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.

Applications for Mouse/Rat CCR8 Antibody

Application
Recommended Usage

Western Blot

0.5 µg/mL
Sample: Mouse thymus tissue and rat thymus tissue
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Reconstitution Buffer Available:
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Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CCR8

CCR8 (C-C chemokine receptor 8; also CD198) is a 41‑43 kDa member of the GPCR #1 family of transmembrane proteins. Mouse CCR8 is expressed on vascular smooth muscle cells, monocytes, eosinophils, peritoneal macrophages, thymocytes, CD8+ T cells, Langerhans cells and neurons. CCL1/TCA3 and vMIP-1 are known agonists for CCR8. Mouse CCR8 is a 7-transmembrane protein that is 353 amino acids (aa) in length. It contains a 33 aa N-terminal extracellular domain plus a 50 aa C-terminal cytoplasmic tail. In mouse, CCR8 is N- and possibly O‑glycosylated, and known to be sulfated on Tyr14 and 15. The unusual nature of these posttranslational modifications may lead to anomalous migration in SDS-PAGE. Over aa sequences 1‑33 and 92‑105 collectively, mouse CCR8 shares 64% and 85% aa identity with human and rat CCR8, respectively.

Alternate Names

CCR8, CD198, CDw198, ChemR1, CKRL1, GPRCL1, TER-1

Entrez Gene IDs

1237 (Human); 12776 (Mouse)

Gene Symbol

CCR8

UniProt

P56484

Additional CCR8 Products

Product Documents for Mouse/Rat CCR8 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Mouse/Rat CCR8 Antibody

For research use only

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