Mouse/Rat FABP1/L-FABP Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB1565
Key Product Details
Species Reactivity
Validated:
Mouse, Rat
Cited:
Mouse
Applications
Validated:
Immunohistochemistry, Simple Western, Western Blot
Cited:
Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2B Clone # 220119
Product Specifications
Immunogen
E. coli-derived recombinant rat FABP1/L‑FABP
Met1-Ile127
Accession # P02692
Met1-Ile127
Accession # P02692
Specificity
Detects rat FABP1/L‑FABP in direct ELISAs and Western blots. In direct ELISAs and Western blots no cross-reactivity with recombinant rat FABP2, recombinant human FABP3, recombinant mouse (rm) FABP4, or rmFABP5 is observed.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2B
Scientific Data Images for Mouse/Rat FABP1/L-FABP Antibody
Detection of Mouse FABP1/L‑FABP by Western Blot.
Western blot shows lysates of mouse liver tissue. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Mouse/ Rat FABP1/L-FABP Monoclonal Antibody (Catalog # MAB1565) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for FABP1/L-FABP at approximately 13 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Detection of Rat FABP1/L‑FABP by Western Blot.
Western blot shows lysate of rat liver tissue. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Mouse/Rat FABP1/L-FABP Monoclonal Antibody (Catalog # MAB1565) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for FABP1/L-FABP at approximately 13 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Detection of Mouse FABP1/L‑FABP by Simple WesternTM.
Simple Western lane view shows lysates of mouse liver tissue, loaded at 0.2 mg/mL. A specific band was detected for FABP1/L‑FABP at approximately 16 kDa (as indicated) using 10 µg/mL of Mouse Anti-Mouse/Rat FABP1/L‑FABP Monoclonal Antibody (Catalog # MAB1565). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.Applications for Mouse/Rat FABP1/L-FABP Antibody
Application
Recommended Usage
Immunohistochemistry
8-25 µg/mL
Sample: Perfusion fixed frozen sections of rat liver
Sample: Perfusion fixed frozen sections of rat liver
Simple Western
10 µg/mL
Sample: Mouse liver tissue
Sample: Mouse liver tissue
Western Blot
2 µg/mL
Sample: Mouse and rat liver tissue
Sample: Mouse and rat liver tissue
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: FABP1/L-FABP
Long Name
Fatty Acid-Binding Protein 1
Alternate Names
L-FABP, LFABP
Gene Symbol
FABP1
UniProt
Additional FABP1/L-FABP Products
Product Documents for Mouse/Rat FABP1/L-FABP Antibody
Product Specific Notices for Mouse/Rat FABP1/L-FABP Antibody
For research use only
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