Mouse/Rat GABA-A R alpha 5 C-Terminus Antibody

R&D Systems, part of Bio-Techne | Catalog # PPS027

R&D Systems, part of Bio-Techne
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PPS027
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Key Product Details

Species Reactivity

Validated:

Mouse, Rat

Cited:

Rat

Applications

Validated:

Western Blot

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

View all GABA-A R alpha 5 Antibodies »

Product Specifications

Immunogen

Fusion protein from the cytosolic loop of the rat GABAA R alpha5 subunit, C-Terminus

Specificity

Mouse and rat ~55 kDa GABAA R alpha5 subunit, C-Terminus

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Mouse/Rat GABA-A R alpha 5 C-Terminus Antibody

Detection of GABAAR a5 C-Terminus antibody by Western Blot.

Detection of GABAAR alpha5 C-Terminus by Western Blot.

Western blot of 7 μg of rat hippocampus showing specific immunolabeling of the ~55 kDa alpha5 subunit of the GABAAR.

Applications for Mouse/Rat GABA-A R alpha 5 C-Terminus Antibody

Application
Recommended Usage

Western Blot

1:1000 dilution
Sample: Rat brain (hippocampus) tissue

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

100 μL in 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 μg/mL BSA and 50% glycerol.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

For long-term storage, ≤ -20° C is recommended. Product is stable at ≤ -20° C for at least 1 year.

Background: GABA-A R alpha 5

GABAA ( gamma-aminobutyric acid-type A) receptors are members of the cysteine-loop family of neurotransmitter-gated ion channels. GABA binding to A-type receptors induces anion-selective ion channel opening. These receptors are the principal fast inhibitory neurotransmitter receptors in the CNS. GABAA receptors are heteropentamer combinations of seven subunit types; alpha, beta, gamma, delta, epsilon, theta, and π. Three subunits, alpha, beta, and gamma, have at least three separate gene products in mammals, and typical GABAA receptors have some combination of alpha, beta and gamma subunits. The rat alpha5 isoform is a 439 amino acid (aa), 4 transmembrane protein with two terminal extracellular regions. The ligand-binding region is in the N-terminus (aa 25 - 232). The alpha5 subunit is unusual in that it contains an N-terminal histidine which renders it very sensitive to zinc. It is also associated with extrasynaptic sites and seems to mediate tonic inhibition in hippocampal neurons.

References

  1. Darlison, M.G. et al. (2005) Cell. Mol. Neurobiol. 25:607.
  2. Akabas, M.H. (2004) Int. Rev. Neurobiol. 62:1.
  3. Caraiscos, V.B. et al. (2004) Proc. Natl. Acad. Sci. USA 101:3662.
  4. Fisher, J.L. (2002) Neuropharmacology 42:922.
  5. Loebrich, S. et al. (2006) EMBO J. 25:987.

Long Name

gamma-Aminobutyric Acid Type A Receptor, alpha-5 Polypeptide

Alternate Names

GABAAR alpha 5, GABAARa5, GABRA5

Entrez Gene IDs

2558 (Human); 110886 (Mouse); 29707 (Rat)

Gene Symbol

GABRA5

Additional GABA-A R alpha 5 Products

Product Documents for Mouse/Rat GABA-A R alpha 5 C-Terminus Antibody

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Mouse/Rat GABA-A R alpha 5 C-Terminus Antibody

For research use only

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