Mouse/Rat Growth Hormone Biotinylated Antibody
R&D Systems, part of Bio-Techne | Catalog # BAF1566
Key Product Details
Species Reactivity
Mouse, Rat
Applications
Immunohistochemistry, Western Blot
Label
Biotin
Antibody Source
Polyclonal Goat IgG
Product Specifications
Immunogen
E. coli-derived recombinant rat Growth Hormone
Leu27-Phe216
Accession # P01244
Leu27-Phe216
Accession # P01244
Specificity
Detects rat Growth Hormone in Western blots. In this format, approximately 10% cross-reactivity with recombinant human Growth Hormone is observed.
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Scientific Data Images for Mouse/Rat Growth Hormone Biotinylated Antibody
Growth Hormone in Rat Pituitary.
Growth Hormone was detected in perfusion fixed frozen sections of rat pituitary using Goat Anti-Rat Growth Hormone Biotinylated Antigen Affinity-purified Polyclonal Antibody (Catalog # BAF1566) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Lower panel shows a lack of labeling if primary antibodies are omitted and tissue is stained only with secondary antibody followed by incubation with detection reagents. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.Growth Hormone in Mouse Embryo.
Growth Hormone was detected in immersion fixed frozen sections of mouse embryo using Goat Anti-Rat Growth Hormone Biotinylated Antigen Affinity-purified Polyclonal Antibody (Catalog # BAF1566) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Lower panel shows a lack of labeling if primary antibodies are omitted and tissue is stained only with secondary antibody followed by incubation with detection reagents. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.Applications for Mouse/Rat Growth Hormone Biotinylated Antibody
Application
Recommended Usage
Immunohistochemistry
5-15 µg/mL
Sample: Immersion fixed frozen sections of mouse embryo (E13-15) and perfusion fixed frozen sections of rat pituitary
Sample: Immersion fixed frozen sections of mouse embryo (E13-15) and perfusion fixed frozen sections of rat pituitary
Western Blot
0.1 µg/mL
Sample: Recombinant Rat Growth Hormone
Sample: Recombinant Rat Growth Hormone
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Growth Hormone
Alternate Names
GH-N, GH1, GHB5, GHN, IGHD1A, IGHD2, Somatotropin
Entrez Gene IDs
2688 (Human)
Gene Symbol
GH1
UniProt
Additional Growth Hormone Products
Product Documents for Mouse/Rat Growth Hormone Biotinylated Antibody
Product Specific Notices for Mouse/Rat Growth Hormone Biotinylated Antibody
For research use only
Loading...
Loading...
Loading...
Loading...