Mouse/Rat ICAM-5 Antibody
R&D Systems, part of Bio-Techne | Catalog # AF1173
Key Product Details
Species Reactivity
Validated:
Mouse, Rat
Cited:
Mouse, Rat
Applications
Validated:
Immunohistochemistry, Western Blot
Cited:
Immunoprecipitation, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Goat IgG
Product Specifications
Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse ICAM‑5
Leu31-Arg828 (Pro47Arg)
Accession # Q60625
Leu31-Arg828 (Pro47Arg)
Accession # Q60625
Specificity
Detects mouse ICAM-5 in direct ELISAs. Detects mouse and rat ICAM-5 in Western blots. In direct ELISAs, approximately 10% cross-reactivity with recombinant human (rh) ICAM-5 and recombinant mouse (rm) ICAM-2 is observed, 5% cross-reactivity with recombinant rat ICAM-1, rhICAM-1, and rhICAM-3 is observed and less than 1% cross-reactivity with rmICAM-1 and rmVCAM-1 is observed.
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Scientific Data Images for Mouse/Rat ICAM-5 Antibody
Detection of Mouse and Rat ICAM‑5 by Western Blot.
Western blot shows lysates of rat olfactory bulb tissue, mouse olfactory bulb tissue, mouse brain tissue, and human brain (cerebellum) tissue. PVDF membrane was probed with 0.2 µg/mL of Goat Anti-Mouse ICAM-5 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1173) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for ICAM-5 at approximately 150 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.Applications for Mouse/Rat ICAM-5 Antibody
Application
Recommended Usage
Immunohistochemistry
5-15 µg/mL
Sample: Perfusion fixed frozen sections of mouse brain (hippocampus and cortex)
Sample: Perfusion fixed frozen sections of mouse brain (hippocampus and cortex)
Western Blot
0.2 µg/mL
Sample: Rat olfactory bulb tissue, mouse olfactory bulb tissue, and mouse brain tissue
Sample: Rat olfactory bulb tissue, mouse olfactory bulb tissue, and mouse brain tissue
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: ICAM-5
ICAM‑5 to bind LFA-1 suggests that ICAM-5 may play an important role in immune responses in the central nervous system (2). Additionally, ICAM-5 has been found to promote homophilic binding via binding of the first Ig domain to Ig domains 4-5. Homophilic adhesion activity of ICAM-5 is regulated by a monomer/tetramer transition. ICAM-5 expression temporally parallels the onset of dendritic elongation and synaptogenesis during the postnatal period suggesting that ICAM-5 may provide a brain segment-specific cue for synaptogenesis or dendrite-dendrite interaction (3).
References
- Tian, L. et al. (2000) Eur. J. Immunol. 30:810.
- Mizuno, T. and K. Mori (1997) J. Biol. Chem. 272:1156.
- Tian, L. et al. (2000) J. Cell Biol. 150:243.
Long Name
Intercellular Adhesion Molecule 5
Alternate Names
ICAM5, Telencephalin
Gene Symbol
ICAM5
UniProt
Additional ICAM-5 Products
Product Documents for Mouse/Rat ICAM-5 Antibody
Product Specific Notices for Mouse/Rat ICAM-5 Antibody
For research use only
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