Mouse/Rat Inositol Monophosphatase 3/IMPAD1 Antibody
R&D Systems, part of Bio-Techne | Catalog # AF7028
Key Product Details
Species Reactivity
Validated:
Mouse, Rat
Cited:
Mouse
Applications
Validated:
Immunocytochemistry, Western Blot
Cited:
Immunocytochemistry, Immunohistochemistry, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Sheep IgG
Product Specifications
Immunogen
Chinese hamster ovary cell line CHO-derived recombinant mouse Inositol Monophosphatase 3/IMPAD1
Asn145-His356
Accession # Q80V26
Asn145-His356
Accession # Q80V26
Specificity
Detects recombinant mouse Inositol Monophosphatase 3/IMPAD1 in direct ELISAs and Western blots. Detects mouse Inositol Monophosphatase 3/IMPAD1 and rat Inositol Monophosphatase 3/IMPAD1 in Western blots.
Clonality
Polyclonal
Host
Sheep
Isotype
IgG
Scientific Data Images for Mouse/Rat Inositol Monophosphatase 3/IMPAD1 Antibody
Detection of Mouse and Rat Inositol Monophosphatase 3/IMPAD1 by Western Blot.
Western blot shows lysates of Neuro-2A mouse neuroblastoma cell line and PC-12 rat adrenal pheochromocytoma cell line. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Mouse Inositol Monophosphatase 3/IMPAD1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7028) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for Inositol Monophosphatase 3/IMPAD1 at approximately 42 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Inositol Monophosphatase 3/IMPAD1 in Mouse Mesenchymal Stem Cells.
Inositol Monophosphatase 3/IMPAD1 was detected in immersion fixed mouse mesenchymal stem cells differentiated into chondrocytes using Sheep Anti-Mouse/Rat Inositol Monophosphatase 3/IMPAD1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7028) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Sheep IgG Secondary Antibody (red; Catalog # NL010) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.Applications for Mouse/Rat Inositol Monophosphatase 3/IMPAD1 Antibody
Application
Recommended Usage
Immunocytochemistry
5-15 µg/mL
Sample: Immersion fixed mouse mesenchymal stem cells differentiated into chondrocytes
Sample: Immersion fixed mouse mesenchymal stem cells differentiated into chondrocytes
Western Blot
1 µg/mL
Sample: Neuro‑2A mouse neuroblastoma cell line and PC‑12 rat adrenal pheochromocytoma cell line
Sample: Neuro‑2A mouse neuroblastoma cell line and PC‑12 rat adrenal pheochromocytoma cell line
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Sterile PBS to a final concentration of 0.2 mg/mL. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Inositol Monophosphatase 3/IMPAD1
IMPAD1 (Inositol monophosphatase domain-containing protein 1; also IMPA3, gPAPP and IMPase 3) is a 40-42 kDa member of the inositol monophosphatase family of proteins. It is expressed in embryo, and found in Purkinje cells, brain stem, lung and chondrocytes. IMPAD1 in theory may catalyze the synthesis of myo-inositol from myo-inositol monophosphate. Free myo-inositol is used to generate inositol phospholipid, an essential component of intracellular signaling pathways that mobilize calcium. IMPAD1 is reported to promote sulfation of chondroitin by converting PAP (an endproduct of the sulfation process) to 5'-AMP. PAP is a known inhibitor of SULTs. IMPAD1 is a 356 amino acid (aa) type II transmembrane Golgi-embedded glycoprotein. It contains a short cytoplasmic tail (aa 1-12) and an extended luminal region (aa 34-356) that contains its catalytic domain (aa 60-347). Over aa 145-356, mouse IMPAD1 shares 99% and 93% aa sequence identity with rat and human IMPAD1, respectively.
Alternate Names
IMP3, IMPA3, IMPase 3
Gene Symbol
BPNT2
UniProt
Additional Inositol Monophosphatase 3/IMPAD1 Products
Product Documents for Mouse/Rat Inositol Monophosphatase 3/IMPAD1 Antibody
Product Specific Notices for Mouse/Rat Inositol Monophosphatase 3/IMPAD1 Antibody
For research use only
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