Mouse/Rat M-Cadherin/Cadherin-15 Antibody
R&D Systems, part of Bio-Techne | Catalog # AF7677
Key Product Details
Species Reactivity
Validated:
Mouse, Rat
Cited:
Mouse
Applications
Validated:
Immunohistochemistry, Western Blot
Cited:
Immunohistochemistry
Label
Unconjugated
Antibody Source
Polyclonal Sheep IgG
Product Specifications
Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse M‑Cadherin/Cadherin‑15
Val22-Ala605 (Arg159His)
Accession # P33146
Val22-Ala605 (Arg159His)
Accession # P33146
Specificity
Detects mouse and rat M‑Cadherin/Cadherin‑15 in direct ELISAs and Western blots. In direct ELISAs, approximately 10% cross-reactivity with recombinant human M‑Cadherin/Cadherin‑15 is observed, and less than 1% cross-reactivity with recombinant mouse P-Cadherin is observed.
Clonality
Polyclonal
Host
Sheep
Isotype
IgG
Scientific Data Images for Mouse/Rat M-Cadherin/Cadherin-15 Antibody
Detection of Mouse and Rat M‑Cadherin/ Cadherin‑15 by Western Blot.
Western blot shows lysates of C2C12 mouse myoblast cell line, mouse brain (cerebellum) tissue, and L6 rat myoblast cell line. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Mouse/Rat M-Cadherin/Cadherin-15 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7677) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for M-Cadherin/Cadherin-15 at approximately 125 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
M‑Cadherin/Cadherin‑15 in Human Embryonic Muscle.
M-Cadherin/Cadherin-15 was detected in perfusion fixed frozen sections of human embryonic muscle using Sheep Anti-Mouse/Rat M-Cadherin/Cadherin-15 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7677) at 1.7 µg/mL overnight at 4 °C. Tissue was stained using the NorthernLights™ 557-conjugated Anti-Sheep IgG Secondary Antibody (red; Catalog # NL010) and counterstained with DAPI (blue). Specific staining was localized to plasma membrane. View our protocol for Fluorescent IHC Staining of Frozen Tissue Sections.Applications for Mouse/Rat M-Cadherin/Cadherin-15 Antibody
Application
Recommended Usage
Immunohistochemistry
1-25 µg/mL
Sample: Perfusion fixed frozen sections of human embryonic muscle
Sample: Perfusion fixed frozen sections of human embryonic muscle
Western Blot
1 µg/mL
Sample: C2C12 mouse myoblast cell line, mouse brain (cerebellum) tissue, and L6 rat myoblast cell line
Sample: C2C12 mouse myoblast cell line, mouse brain (cerebellum) tissue, and L6 rat myoblast cell line
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Sterile PBS to a final concentration of 0.2 mg/mL. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: M-Cadherin/Cadherin-15
CDH-15 (Cadherin 15; also M-cadherin, Muscle cadherin and Cadherin-14) is a 125-127 kDa atypical member of the classical cadherin family, cadherin superfamily of molecules. It is expressed by muscle satellite cells, cells of the embryonic myotome, and hematopoietic bone marrow stem cells. CDH-15 appears to bind homotypically in trans, thus allowing for the identification and subsequent fusion of myoblast precursors, particularly those in slow-twitch (or red fiber) muscles. This is accompanied by a downregulation of mitochondrial induced apoptosis. Mouse CDH-15 is synthesized as a 784 amino acid (aa) preproprecursor. It contains a 21 aa signal sequence, a 38 aa propeptide, and a 725 aa mature region. The mature region is expressed as a type I transmembrane glycoprotein that possesses a 546 aa extracellular region (aa 60-605) and a 159 aa cytoplasmic domain (aa 626-784). The extracellular region shows five consecutive cadherin domains. Over aa 22-605, mouse CDH-15 shares 88% and 97% aa sequence identity with human and rat CDH-15, respectively.
Long Name
M-Cadherin [Myotubule]
Alternate Names
Cadherin-15, CDH14, CDH15, CDHM, MCAD, MCadherin
Gene Symbol
CDH15
UniProt
Additional M-Cadherin/Cadherin-15 Products
Product Documents for Mouse/Rat M-Cadherin/Cadherin-15 Antibody
Product Specific Notices for Mouse/Rat M-Cadherin/Cadherin-15 Antibody
For research use only
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