Mouse/Rat Neuropilin-1 Alexa Fluor® 700-conjugated Antibody
R&D Systems, part of Bio-Techne | Catalog # FAB566N
Key Product Details
Species Reactivity
Validated:
Mouse, Rat
Cited:
Mouse
Applications
Validated:
Flow Cytometry
Cited:
Flow Cytometry
Label
Alexa Fluor 700 (Excitation = 675-700 nm, Emission = 723 nm)
Antibody Source
Polyclonal Goat IgG
Product Specifications
Immunogen
Mouse myeloma cell line NS0-derived recombinant rat Neuropilin-1
Phe22-Ala810 (Lys811Arg), Ser829-Asp854
Accession # Q9QWJ9
Phe22-Ala810 (Lys811Arg), Ser829-Asp854
Accession # Q9QWJ9
Specificity
Detects mouse and rat Neuropilin-1 in direct ELISAs and Western blots. In direct ELISAs, approximately 8% cross-reactivity with recombinant human Neuropilin‑1 is observed, and less than 1% cross-reactivity with recombinant rat Neuropilin-2 is observed.
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Scientific Data Images for Mouse/Rat Neuropilin-1 Alexa Fluor® 700-conjugated Antibody
Detection of Neuropilin‑1 in bEnd.3 Mouse Cell Line by Flow Cytometry.
bEnd.3 mouse endothelioma cell line was stained with Goat Anti-Mouse/Rat Neuropilin-1 Alexa Fluor® 700-conjugated Antigen Affinity-purified Polyclonal Antibody (Catalog # FAB566N, filled histogram) or isotype control antibody (Catalog # IC108N, open histogram). View our protocol for Staining Membrane-associated Proteins.
Detection of Neuropilin‑1 in PC‑12 Rat Cell Line by Flow Cytometry.
PC-12 rat adrenal pheochromocytoma cell line was stained with Goat Anti-Mouse/Rat Neuropilin-1 Alexa Fluor® 700-conjugated Antigen Affinity-purified Polyclonal Antibody (Catalog # FAB566N, filled histogram) or isotype control antibody (Catalog # IC108N, open histogram). View our protocol for Staining Membrane-associated Proteins.
Detection of Mouse Neuropilin-1 by Flow Cytometry
pTregs show a higher susceptibility to convert into exFoxp3 cells after transfer into dry eye hosts. pTregs (CD4+CD25+Nrp-1−) and tTregs (CD4+CD25+Nrp-1+) from transgenic dry eye disease (DED) mice (Foxp3-GFP×R26-RFP) were adoptively transferred into control and DED transplant recipients. (A and B) Draining lymph nodes were harvested at day 14 post-transplantation. Single cell suspensions were analyzed by flow cytometry. Frequencies of exFoxp3 cells (GFP−RFP+) from control and DED hosts that received (A) pTregs (59.02 ± 0.92% vs. 78.26 ± 0.69%, n = 4, ***p < 0.0001 unpaired two-tailed Student’s t test) or (B) tTregs was assessed. (C) Representative flow cytometry plots showing frequencies of IL-17 and IFN gamma-expressing exFoxp3 cells in draining lymph nodes from control and dry eye hosts. (D) Nrp1+ tTregs or Nrp1− pTregs from hosts with accepted allografts were adoptively transferred to dry eye hosts, and graft survival was assessed for 8 weeks (n = 10, χ2 = 0.6087, p = 0.4353). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/29728574), licensed under a CC-BY license. Not internally tested by R&D Systems.Applications for Mouse/Rat Neuropilin-1 Alexa Fluor® 700-conjugated Antibody
Application
Recommended Usage
Flow Cytometry
5 µL/106 cells
Sample: bEnd.3 mouse endothelioma cell line and PC‑12 rat adrenal pheochromocytoma cell line
Sample: bEnd.3 mouse endothelioma cell line and PC‑12 rat adrenal pheochromocytoma cell line
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Formulation
Supplied in a saline solution containing BSA and Sodium Azide.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
- 12 months from date of receipt, 2 to 8 °C as supplied.
Background: Neuropilin-1
Neuropilin-1 is a type I transmembrane protein that is expressed in the developing nervous system and by endothelial and tumor cells. Neuropilin-1 binds members of the class III secreted semaphorin subfamily as well as some isoforms of VEGF family proteins. The amino acid sequence of rat Neuropilin-1 extracellular domain is 98% and 93% identical to that of mouse and human Neuropilin-1, respectively.
Alternate Names
BDCA-4, CD304, Neuropilin1, NRP1
Gene Symbol
NRP1
UniProt
Additional Neuropilin-1 Products
Product Specific Notices for Mouse/Rat Neuropilin-1 Alexa Fluor® 700-conjugated Antibody
* This product or the use of this product is covered by U.S. Patents owned by The Regents of the University of California. This product is for research use only and is not to be used for commercial purposes. Use of this product to produce products for sale or for diagnostic, therapeutic or drug discovery purposes is prohibited. In order to obtain a license to use this product for such purposes, contact The Regents of the University of California.
U.S. Patent # 6,054,293, 6,623,738, and other U.S. and international patents pending.
This product is provided under an agreement between Life Technologies Corporation and R&D Systems, Inc, and the manufacture, use, sale or import of this product is subject to one or more US patents and corresponding non-US equivalents, owned by Life Technologies Corporation and its affiliates. The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The sale of this product is expressly conditioned on the buyer not using the product or its components (1) in manufacturing; (2) to provide a service, information, or data to an unaffiliated third party for payment; (3) for therapeutic, diagnostic or prophylactic purposes; (4) to resell, sell, or otherwise transfer this product or its components to any third party, or for any other commercial purpose. Life Technologies Corporation will not assert a claim against the buyer of the infringement of the above patents based on the manufacture, use or sale of a commercial product developed in research by the buyer in which this product or its components was employed, provided that neither this product nor any of its components was used in the manufacture of such product. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, Cell Analysis Business Unit, Business Development, 29851 Willow Creek Road, Eugene, OR 97402, Tel: (541) 465-8300. Fax: (541) 335-0354.
For research use only
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