Mouse/Rat PDZK1 Antibody
R&D Systems, part of Bio-Techne | Catalog # AF5780
Key Product Details
Species Reactivity
Mouse, Rat
Applications
Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Goat IgG
Product Specifications
Immunogen
E. coli-derived recombinant mouse PDZK1
aa 1-519
Accession # Q9JIL4
aa 1-519
Accession # Q9JIL4
Specificity
Detects mouse and rat PDZK1 in Western blots.
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Scientific Data Images for Mouse/Rat PDZK1 Antibody
Detection of Mouse and Rat PDZK1 by Western Blot.
Western blot shows lysates of mouse liver tissue and rat liver tissue. PVDF Membrane was probed with 1 µg/mL of Goat Anti-Mouse/Rat PDZK1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5780) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for PDZK1 at approximately 75 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.Applications for Mouse/Rat PDZK1 Antibody
Application
Recommended Usage
Western Blot
1 µg/mL
Sample: Mouse liver tissue and rat liver tissue
Sample: Mouse liver tissue and rat liver tissue
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: PDZK1
PDZK1 (PDZ Domain-containing 1; also CFTR-associated protein of 70 kDa/CAP70) is a 65‑70 kDa member of the NHER (Na/H Exchange Regulator) family of proteins. It is only expressed in epithelium, particularly that found in intestine, kidney and liver. PDZK1 serves as a scaffold protein that connects cell membrane proteins to cytoplasmic regulators. It also serves a critical role in basal-level bicarbonate secretion. Mouse PDZK1 is 519 amino acids (aa) in length and contains four PDZ domains (aa 9‑90; 128‑215; 243‑323; and 378‑458) plus three phosphorylation sites at Ser489, Ser492 and Ser514. Mouse PDZK1 shares 78% and 91% aa identity with human and rat PDZK1, respectively.
Long Name
PDZ Domain Containing 1
Alternate Names
CAP70, CLAMP, NaPi-Cap1, NHERF3, PDZD1
Gene Symbol
PDZK1
UniProt
Additional PDZK1 Products
Product Documents for Mouse/Rat PDZK1 Antibody
Product Specific Notices for Mouse/Rat PDZK1 Antibody
For research use only
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