Mouse/Rat Phospho-Potassium Channel Kv3.1 (S503) Antibody

Name: Mouse/Rat Phospho-Potassium Channel Kv3.1 (S503) Antibody
Brand: R&D Systems
SKU: PPS016

R&D Systems, part of Bio-Techne | Catalog # PPS016

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Product Datasheet / COA / SDS

Voltage Gated

100% Guarantee

Product Specifications
Applications
Preparation & Storage
Background
Product Documents
Specific Notices

Key Product Details

Species Reactivity

Mouse, Rat

Applications

Immunohistochemistry, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

View all Potassium Channel Kv3.1 Antibodies »

Product Specifications

Immunogen

Phosphopeptide corresponding to amino acid residues surrounding the phospho-S503 of Potassium Channel Kv3.1

Specificity

Rat ~100k Voltage Gated Potassium Channel KV3.1-subunit protein phosphorylated at S503

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Applications for Mouse/Rat Phospho-Potassium Channel Kv3.1 (S503) Antibody

Application

Recommended Usage

Immunohistochemistry

1:1000 dilution
Sample: Frozen sections; unpublished observations

Western Blot

1:1000 dilution
Sample: Rat brain tissue

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

100 μL in 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 μg/mL BSA and 50% glycerol.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

For long-term storage, ≤ -20° C is recommended. Product is stable at ≤ -20° C for at least 1 year.

Background: Potassium Channel Kv3.1

Rat Kv3.1 is a 98 - 110 kDa, six transmembrane glycoprotein that belongs to the C subfamily of the potassium channel family. It generates a voltage-dependent channel selective for potassium that helps determine the electrical property of GABAergic and auditory neurons. The symbol K stands for potassium, v for the molecule’s ligand (i.e. voltage), 3 for the family type that is determined by similarity to fruitfly genes, and 1 for the gene number within the family. There are four family types (Kv1-4) that generate homo- and heterotetramers with themselves and each other. Kv3 family members are associated with very fast activation and deactivation rates of firing. Kv3.1 has two splice forms which differ at their intracellular C-terminus. There is a 98 kDa, 511 amino acid (aa) Kv3.1a, and a 110 kDa, 585 aa Kv3.1b. The two forms may coexist but possibly not multimerize. Kv3.1b has a serine at position 503 that is not shared by Kv3.1a. Phosphorylation by PKC at this site disrupts typical high frequency firing, but generates a coordinated intermediate frequency firing when presented with an intermediate frequency stimulus.

References

Trimmer, J.S. and K.J. Rhodes (2004) Annu. Rev. Physiol. 66:477.
Rudy, B. et al. (1999) Ann. N.Y. Acad. Sci. 868:304.
Macica, C.M. et al. (2003) J. Neurosci. 23:1133.
Rettig, J. et al. (1992) EMBO J. 11:2473.

Alternate Names

KCNC1

Entrez Gene IDs

3746 (Human); 16502 (Mouse)

Gene Symbol