Mouse/Rat PTP1B Antibody
R&D Systems, part of Bio-Techne | Catalog # AF3954
Key Product Details
Species Reactivity
Validated:
Mouse, Rat
Cited:
Human, Mouse, Rat
Applications
Validated:
Simple Western, Western Blot
Cited:
Immunohistochemistry, Immunohistochemistry-Frozen, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Goat IgG
Product Specifications
Immunogen
E. coli-derived recombinant mouse PTP1B
Met1-Phe431
Accession # P35821
Met1-Phe431
Accession # P35821
Specificity
Detects mouse and rat PTP1B in Western blots. In Western blots, less than 1% cross-reactivity with recombinant human PTP1B is observed.
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Scientific Data Images for Mouse/Rat PTP1B Antibody
Detection of Mouse/Rat PTP1B by Western Blot.
Western blot shows lysates of Neuro-2A mouse neuroblastoma cell line. PVDF membrane was probed with 0.3 µg/mL Goat Anti-Mouse/Rat PTP1B Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3954) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). For additional reference, Recombinant Human PTP1B (Catalog # 1366-PT) and recombinant mouse PTP1B 5 ng/lane) were included. A specific band for PTP1B was detected at approximately 50 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Detection of Mouse PTP1B by Simple WesternTM.
Simple Western lane view shows lysates of Neuro-2A mouse neuroblastoma cell line, loaded at 0.2 mg/mL. A specific band was detected for PTP1B at approximately 56 kDa (as indicated) using 5 µg/mL of Goat Anti-Mouse/Rat PTP1B Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3954) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.Applications for Mouse/Rat PTP1B Antibody
Application
Recommended Usage
Simple Western
5 µg/mL
Sample: Neuro‑2A mouse neuroblastoma cell line
Sample: Neuro‑2A mouse neuroblastoma cell line
Western Blot
0.3 µg/mL
Sample: Neuro-2A mouse neuroblastoma cell line
Sample: Neuro-2A mouse neuroblastoma cell line
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: PTP1B
Protein tyrosine phosphatase 1B (PTP1B), also known as protein tyrosine phosphatase, non-receptor type 1 (PTPN1) and placental protein tyrosine phosphatase, dephosphorylates tyrosine residues in proteins. PTP1B is a cytosolic enzyme anchored in the endoplasmic reticulum that plays a critical role in regulating growth factor responsiveness. PTP1B knockout mice show increased resistance to high fat diet-induced obesity and have exaggerated responses to insulin.
Long Name
Protein Tyrosine Phosphatase 1B
Alternate Names
PTPN1
Gene Symbol
PTPN1
UniProt
Additional PTP1B Products
Product Documents for Mouse/Rat PTP1B Antibody
Product Specific Notices for Mouse/Rat PTP1B Antibody
For research use only
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