Mouse/Rat RIBP/SH2D2A Antibody

R&D Systems, part of Bio-Techne | Catalog # AF6265

R&D Systems, part of Bio-Techne
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AF6265
AF6265-SP
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Key Product Details

Species Reactivity

Mouse, Rat

Applications

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Sheep IgG

View all SH2D2A Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant mouse RIBP/SH2D2A
Ile299-Ser374
Accession # Q9QXK9

Specificity

Detects mouse and rat RIBP/SH2D2A in Western blots. 

Clonality

Polyclonal

Host

Sheep

Isotype

IgG

Scientific Data Images for Mouse/Rat RIBP/SH2D2A Antibody

Detection of Mouse and Rat RIBP/SH2D2A antibody by Western Blot.

Detection of Mouse and Rat RIBP/SH2D2A by Western Blot.

Western blot shows lysates of CTLL-2 mouse cytotoxic T cell line and Nb2-11 rat lymphoma cell line. PVDF Membrane was probed with 1 µg/mL of Sheep Anti-Mouse/Rat RIBP/SH2D2A Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6265) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for RIBP/SH2D2A at approximately 46 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Applications for Mouse/Rat RIBP/SH2D2A Antibody

Application
Recommended Usage

Western Blot

1 µg/mL
Sample: CTLL‑2 mouse cytotoxic T cell line and Nb2‑11 rat lymphoma cell line

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Reconstitution Buffer Available:
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Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: SH2D2A

RIBP (Rlk/Itk‑binding protein; also SH2D2A, LAD/Lck‑associated adapter protein, MEKK2‑binding protein and TSAd) is a 45‑47 kDa cytoplasmic adaptor protein. It is found in B cells, thymocytes, NK cells and T cells, and appears to be necessary for TCR‑initiated proliferation, plus IL‑2 and IFN‑ gamma secretion. This is likely to be a function of its association with MEKK2 near the T cell receptor interaction site. Mouse RIBP is 374 amino acids (aa) in length and contains one SH2 domain (aa 116‑207), plus a Pro‑rich region (aa 267‑308) that contains an SH3‑binding motif (aa 267‑273). Phosphorylation may occur on Ser237, Ser316 and Tyr325. There is one alternative splice form termed LAD that shows a deletion of aa 254‑261. Over aa 299‑374, mouse RIBP shares 79% and 63% aa identity with rat and the presumed human RIBP ortholog (termed TSAd/SH2D2A), respectively.

Alternate Names

F2771, RIBP, SCAP, TSAd, VRAP

Entrez Gene IDs

9047 (Human); 27371 (Mouse); 310688 (Rat)

Gene Symbol

SH2D2A

UniProt

Q9QXK9

Additional SH2D2A Products

Product Documents for Mouse/Rat RIBP/SH2D2A Antibody

Product Datasheets

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Mouse/Rat RIBP/SH2D2A Antibody

For research use only

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