Mouse/Rat SIGNR3/CD209d Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB7220
Key Product Details
Species Reactivity
Validated:
Mouse, Rat
Applications
Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Rat IgG2A Clone # 728804
Product Summary for Mouse/Rat SIGNR3/CD209d Antibody
Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse SIGNR3/CD209d
Glu82-Lys237
Accession # Q91ZW8
Glu82-Lys237
Accession # Q91ZW8
Specificity
Detects mouse and rat SIGNR3/CD209d in Western blots. In Western blots, 100% cross-reactivity with
recombinant mouse (rm) SIGNR1, 50% cross-reactivity with rmSIGNR7, and no
cross-reactivity with rmSIGNR4 is observed. In direct ELISAs, no cross-reactivity with recombinant human (rh) DC-SIGN, rhDC-SIGNR, rmSIGNR1, rmSIGNR4, or rmSIGNR7 is observed.
Clonality
Monoclonal
Host
Rat
Isotype
IgG2A
Scientific Data Images for Mouse/Rat SIGNR3/CD209d Antibody
Detection of Mouse and Rat SIGNR3/CD209d by Western Blot.
Western blot shows lysates of J774A.1 mouse reticulum cell sarcoma macrophage cell line and NR8383 rat alveolar macrophage cell line. PVDF membrane was probed with 1 µg/mL of Rat Anti-Mouse/Rat SIGNR3/CD209d Monoclonal Antibody (Catalog # MAB7220) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody. A specific band was detected for SIGNR3/CD209d at approximately 32 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.Applications for Mouse/Rat SIGNR3/CD209d Antibody
Application
Recommended Usage
Western Blot
1 µg/mL
Sample: J774A.1 mouse reticulum cell sarcoma macrophage cell line and NR8383 rat alveolar macrophage cell line
Sample: J774A.1 mouse reticulum cell sarcoma macrophage cell line and NR8383 rat alveolar macrophage cell line
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Sterile PBS to a final concentration of 0.5 mg/mL. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: SIGNR3/CD209d
Long Name
DC-SIGN-related Protein 3
Alternate Names
CD209d
Gene Symbol
CD209D
UniProt
Additional SIGNR3/CD209d Products
Product Documents for Mouse/Rat SIGNR3/CD209d Antibody
Product Specific Notices for Mouse/Rat SIGNR3/CD209d Antibody
For research use only
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