Mouse/Rat Transglutaminase 2/TGM2 Biotinylated Antibody
R&D Systems, part of Bio-Techne | Catalog # BAF5418
Key Product Details
Species Reactivity
Mouse, Rat
Applications
Western Blot
Label
Biotin
Antibody Source
Polyclonal Sheep IgG
Product Specifications
Immunogen
S. frugiperda insect ovarian cell line Sf 21-derived recombinant mouse Transglutaminase 2/TGM2
Ala2-Ala686
Accession # P21981
Ala2-Ala686
Accession # P21981
Specificity
Detects mouse and rat Transglutaminase 2/TGM2 in Western blots. In Western blots, approximately 10% cross‑reactivity with recombinant human (rh) TGM2 and rhTGM7 is observed, and less than 1% cross‑reactivity with rhTGM3 and rhTGM4 is observed.
Clonality
Polyclonal
Host
Sheep
Isotype
IgG
Applications for Mouse/Rat Transglutaminase 2/TGM2 Biotinylated Antibody
Application
Recommended Usage
Western Blot
0.1 µg/mL
Sample: Recombinant Mouse Transglutaminase 2/TGM2 (Catalog # 5418-TG)
Sample: Recombinant Mouse Transglutaminase 2/TGM2 (Catalog # 5418-TG)
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Transglutaminase 2/TGM2
Transglutaminase 2 (TG2), encoded by the Tgm2 gene, is also known as tissue Transglutaminase (tTG), Transglutaminase C (TGC), and protein-glutamine-gamma -glutamyltransferase. It belongs to the family of transglutaminases that catalyze the posttranslational modification of proteins via calcium dependent cross-linking reactions (1-3). In addition to its function in protein cross-linking, TGM2 is also capable of hydrolyzing both GTP and ATP (4) and has intrinsic kinase activity (5). TGM2 has been implicated in a variety of human diseases including celiac disease, inclusion body myositis, atherosclerosis, and neurodegenerative diseases (6-7).
References
- Gentile, V. et al. (1991) J. Biol. Chem. 266:478.
- Chen, J.S.K. and Mehta K. (1999) Internat. J. Biochem. Cell Biol. 31:817.
- Griffin, M. et al. (2002) Biochem. J. 368:377.
- Lai, T.S. et al. (1998) J. Biol. Chem. 273:1776.
- Mishra, S. et al. (2007) J. Biol. Chem. 282:18108.
- Kim, S-Y. et al. (2002) Neurochem. Int. 40:85.
- Lesort, M. et al. (2000) Prog. Neurobiol. 61:439.
Alternate Names
TGC, TGM2, Transglutaminase 2, tTG
Gene Symbol
TGM2
UniProt
Additional Transglutaminase 2/TGM2 Products
Product Documents for Mouse/Rat Transglutaminase 2/TGM2 Biotinylated Antibody
Product Specific Notices for Mouse/Rat Transglutaminase 2/TGM2 Biotinylated Antibody
For research use only
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