Mouse/Rat WNK1 Antibody
R&D Systems, part of Bio-Techne | Catalog # AF2849
Key Product Details
Species Reactivity
Validated:
Mouse, Rat
Cited:
Mouse
Applications
Validated:
Western Blot
Cited:
Flow Cytometry, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Goat IgG
Product Specifications
Immunogen
E. coli-derived recombinant rat WNK1
Ser816-Ser1173
Accession # Q9JIH7
Ser816-Ser1173
Accession # Q9JIH7
Specificity
Detects mouse and rat WNK1 in Western blots.
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Scientific Data Images for Mouse/Rat WNK1 Antibody
Detection of Mouse/Rat WNK1 by Western Blot.
Western blot shows lysates of DA3 mouse myeloma cell line. PVDF membrane was probed with 0.5 µg/mL of Goat Anti-Mouse/Rat WNK1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2849) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for WNK1 at approximately 230 kDa (as indicated). This experiment was conducted using Immunoblot Buffer Group 1.Applications for Mouse/Rat WNK1 Antibody
Application
Recommended Usage
Western Blot
0.5 µg/mL
Sample: DA3 mouse myeloma cell line
Sample: DA3 mouse myeloma cell line
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Reviewed Applications
Read 1 review rated 4 using AF2849 in the following applications:
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: WNK1
With No Lysine (K) 1 (WNK1) belongs to a novel family of serine/threonine protein kinases involved in regulating ion transport. The WNKs contain a cysteine instead of the usual lysine in their kinase activation domains. WNK1 mutations have been implicated in Pseudohypoaldosteronism type II (PHAII), an autosomal dominant disorder characterized by hypertension, increased renal salt absorption, and impaired potassium and hydrogen excretion.
Long Name
WNK Lysine-deficient Protein Kinase 1
Alternate Names
KDP, PHA2C, PRKWNK1
Gene Symbol
WNK1
UniProt
Additional WNK1 Products
Product Documents for Mouse/Rat WNK1 Antibody
Product Specific Notices for Mouse/Rat WNK1 Antibody
For research use only
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