Mouse RTVP-1 Antibody
R&D Systems, part of Bio-Techne | Catalog # AF4468
Key Product Details
Species Reactivity
Validated:
Mouse
Cited:
Mouse
Applications
Validated:
Western Blot
Cited:
Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Goat IgG
Product Specifications
Immunogen
E. coli-derived recombinant mouse RTVP-1
Ser16-Thr223
Accession # Q9CWG1
Ser16-Thr223
Accession # Q9CWG1
Specificity
Detects mouse RTVP-1 in Western blots.
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Scientific Data Images for Mouse RTVP-1 Antibody
Detection of Mouse RTVP-1 by Western Blot.
Western blot shows lysates of Balb/3T3 mouse embryonic fibroblast cell line and RAW 264.7 mouse monocyte/macrophage cell line. PVDF membrane was probed with 1 µg/mL of Mouse RTVP-1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4468) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for RTVP-1 at approximately 30 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.Applications for Mouse RTVP-1 Antibody
Application
Recommended Usage
Western Blot
1 µg/mL
Sample: Balb/3T3 mouse embryonic fibroblast cell line and RAW 264.7 mouse monocyte/macrophage cell line
Sample: Balb/3T3 mouse embryonic fibroblast cell line and RAW 264.7 mouse monocyte/macrophage cell line
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: RTVP-1
RTVP-1 (related to testes-specific, vespid, and pathogenesis protein 1) can act as both a tumor suppressor and tumor promoter gene. RTVP-1 is a tumor suppressor gene based on its regulation by p53, induction of apoptosis, growth inhibition, and decreased expression in prostate tumors when compared to normal prostate. RTVP-1 is a tumor promoter is based on evidence that overexpression of RTVP-1 decreased the phosphorylation of c-Jun, increased Bcl-2 expression, and increased MMP2 activity. In addition, RTVP-1 is expressed at high levels in glioblastomas while barely detectable in normal brain/astrocytes. Differences in the levels of cellular and extracellular forms as well as different cellular processing of RTVP-1 may account for these differing roles. Additionally, increased RTVP-1 expression is associated with myelomocytic differentiation in macrophages.
Long Name
Related to Testis-specific Vespid and Pathogenesis Protein 1
Alternate Names
GLIPR1, RTVP1
Gene Symbol
GLIPR1
UniProt
Additional RTVP-1 Products
Product Documents for Mouse RTVP-1 Antibody
Product Specific Notices for Mouse RTVP-1 Antibody
For research use only
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