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Mouse Semaphorin 6A Antibody

R&D Systems, part of Bio-Techne | Catalog # AF1615

R&D Systems, part of Bio-Techne
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AF1615
AF1615-SP

Key Product Details

Species Reactivity

Validated:

Mouse

Cited:

Mouse, Primate - Chlorocebus pygerythrus (Vervet Monkey), Transgenic Mouse

Applications

Validated:

Immunohistochemistry, Western Blot

Cited:

Immunocytochemistry, Immunohistochemistry, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG

Product Specifications

Immunogen

S. frugiperda insect ovarian cell line Sf 21-derived recombinant mouse Semaphorin 6A
Gly19-Thr649
Accession # O35464

Specificity

Detects mouse Semaphorin 6A in direct ELISAs and Western blots. In Western blots, approximately 25% cross‑reactivity with recombinant human (rh) Semaphorin 6A is observed and less than 1% cross-reactivity with rhSemaphorin 3A, rhSemaphorin 3B, recombinant mouse (rm) Semaphorin 3C, rmSemaphorin 3F, and rmSemaphorin 7A is observed.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Mouse Semaphorin 6A Antibody

Detection of Semaphorin 6A in Mouse Intestine.

Semaphorin 6A was detected in immersion fixed paraffin-embedded sections of Mouse Intestine using Goat Anti-Mouse Semaphorin 6A Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1615) at 1.7 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Goat IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC004). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using VisUCyte Antigen Retrieval Reagent-Basic (Catalog # VCTS021). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to intestinal glands. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

Applications for Mouse Semaphorin 6A Antibody

Application
Recommended Usage

Immunohistochemistry

1-15 µg/mL
Sample: Immersion fixed paraffin-embedded sections of Mouse Intestine

Western Blot

0.1 µg/mL
Sample: Recombinant Mouse Semaphorin 6A

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

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Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Semaphorin 6A

Semaphorin 6A is a transmembrane protein belonging to the semaphorin family. It functions as an axon guidance signal (1).

References

  1. Fiore, R. and A.W. Puschel (2003) Frontiers Biosci. 8:484.

Alternate Names

Sema6A

Entrez Gene IDs

57556 (Human); 20358 (Mouse)

Gene Symbol

SEMA6A

UniProt

Additional Semaphorin 6A Products

Product Documents for Mouse Semaphorin 6A Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Mouse Semaphorin 6A Antibody

For research use only

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