Mouse Serpin A3N Antibody
R&D Systems, part of Bio-Techne | Catalog # AF4709
Key Product Details
Species Reactivity
Validated:
Mouse
Cited:
Human, Mouse, Transgenic Mouse
Applications
Validated:
Immunoprecipitation, Western Blot
Cited:
Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Goat IgG
Product Specifications
Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse Serpin A3N
Phe21-Lys418
Accession # Q91WP6
Phe21-Lys418
Accession # Q91WP6
Specificity
Detects mouse Serpin A3N in direct ELISAs and Western blots. In direct ELISAs, less than 2% cross-reactivity with recombinant mouse (rm) Serpin A1, recombinant human Serpin A3 and rmSerpin A5 is observed.
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Applications for Mouse Serpin A3N Antibody
Application
Recommended Usage
Immunoprecipitation
25 µg/mL
Sample: Conditioned cell culture medium spiked with Recombinant Mouse Serpin A3N (Catalog # 4709-PI), see our available Western blot detection antibodies
Sample: Conditioned cell culture medium spiked with Recombinant Mouse Serpin A3N (Catalog # 4709-PI), see our available Western blot detection antibodies
Western Blot
0.1 µg/mL
Sample: Recombinant Mouse Serpin A3N (Catalog # 4709-PI)
Sample: Recombinant Mouse Serpin A3N (Catalog # 4709-PI)
Reviewed Applications
Read 1 review rated 5 using AF4709 in the following applications:
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Serpin A3N
Serpin A3N is a serine protease inhibitor that is structurally related to alpha1-antichymotrypsin encoded by the SERPINA3 gene (1). Serpin A3N is highly expressed in brain, testis, lung, thymus, and spleen (2). Serpin A3N secreted by Sertoli cells may regulate the activity of locally produced Granzyme B (3). Granzyme B inhibition by Serpin A3N may therefore regulate Granzyme B-mediated killing by cytotoxic lymphocytes, providing a means to disable cell-mediated immune responses.
References
- Forsyth, S. et al. (2003) Genomics 81:336.
- Horvath, A. J. et al. (2004) J. Mol. Evol. 59:488.
- Hirst, C. E. et al. (2001) Mol. Hum. Reprod. 7:1133.
Alternate Names
Spi2
Gene Symbol
SERPINA3N
UniProt
Additional Serpin A3N Products
Product Documents for Mouse Serpin A3N Antibody
Product Specific Notices for Mouse Serpin A3N Antibody
For research use only
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