Mouse SEZ6L Antibody
R&D Systems, part of Bio-Techne | Catalog # AF4804
Key Product Details
Species Reactivity
Mouse
Applications
Immunohistochemistry
Label
Unconjugated
Antibody Source
Polyclonal Sheep IgG
Product Specifications
Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse SEZ6L
Glu32-Ser889
Accession # AAH65117
Glu32-Ser889
Accession # AAH65117
Specificity
Detects mouse SEZ6L in direct ELISAs. In direct ELISAs, less than 1% cross-reactivity with recombinant mouse (rm) BSRP-A and rmBSRP-C is observed.
Clonality
Polyclonal
Host
Sheep
Isotype
IgG
Scientific Data Images for Mouse SEZ6L Antibody
SEZ6L in Mouse Brain.
SEZ6L was detected in perfusion fixed frozen sections of mouse brain (hippocampus) using Sheep Anti-Mouse SEZ6L Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4804) at 1.7 µg/mL overnight at 4 °C. Tissue was stained using the NorthernLights™ 557-conjugated Anti-Sheep IgG Secondary Antibody (red; Catalog # NL010) and counterstained with DAPI (blue). Specific staining was localized to neuronal cell bodies and processes. View our protocol for Fluorescent IHC Staining of Frozen Tissue Sections.Applications for Mouse SEZ6L Antibody
Application
Recommended Usage
Immunohistochemistry
5-15 µg/mL
Sample: Perfusion fixed frozen sections of mouse brain (hippocampus)
Sample: Perfusion fixed frozen sections of mouse brain (hippocampus)
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Sterile PBS to a final concentration of 0.2 mg/mL. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: SEZ6L
SEZ6L (Seizure 6-Like protein; also BSRP-B/Brain-specific receptor-like protein B) is a 200-210 kDa member of the SEZ6 family of proteins. It is expressed in the plasma membrane and ER of CNS neurons, particularly cerebellar interneurons plus Purkinje and granule cells. SEZ6L likely regulates Ca++ availability and thus impacts PKC alpha activation and mGluR1 signaling. Mature mouse SEZ6L is a 932 amino acid (aa) type I transmembrane protein. It contains an 866 aa extracellular domain (ECD) (aa 32-897) and a 45 aa cytoplasmic tail. The ECD shows three CUB domains and five short consensus repeats (SCRs). There is one 190-200 kDa splice variant termed BSRP-B that contains a Phe substitution for aa 807-872 of the fifth SCR domain. Over aa 32-889, mouse SEZ6L shares 80% and 97% aa identity with human and rat SEZ6L, respectively.
Long Name
Seizure-related 6 Homolog-like
Alternate Names
Acig1, AIG1, Aig1L, BSRP-B, SEZ6L
Gene Symbol
SEZ6L
UniProt
Additional SEZ6L Products
Product Documents for Mouse SEZ6L Antibody
Product Specific Notices for Mouse SEZ6L Antibody
For research use only
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