Mouse Splenocytes were stained with Anti-Mouse Integrin aX/CD11c Phycoerythrin-conjugated Antibody and either (A) Rat IgG2B Isotype Control (Catalog # MAB0061) or (B) Rat Anti-Mouse Siglec-E Monoclonal Antibody (Catalog # MAB5806) followed by Allophycocyanin-conjugated Anti-Rat IgG Secondary Antibody (Catalog # F0113).

Applications for Mouse Siglec-E Antibody

Application

Recommended Usage

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Flow Cytometry

2.5 µg/10⁶ cells
Sample: Mouse splenocytes

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Sterile PBS to a final concentration of 0.5 mg/mL. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Siglec-E

Mouse Siglec-E (Salic acid binding Ig-like lectin E), also known as Siglecl1, is a 52 kDa (predicted) member of the CD33-related subfamily of Siglecs.

Long Name

Sialic Acid Binding Ig-like Lectin E

Alternate Names

SiglecE

Entrez Gene IDs

83382 (Mouse)

Gene Symbol

SIGLECE

UniProt

Q91Y57

Additional Siglec-E Products

Product Documents for Mouse Siglec-E Antibody

Product Datasheets

Download Product Datasheets

COA

SDS

Download SDS

Product Specific Notices for Mouse Siglec-E Antibody

For research use only

Citations
Reviews

Calculators
Flow Cytometry

Mouse Siglec-E Antibody

R&D Systems, part of Bio-Techne | Catalog # MAB5806

Key Product Details

Species Reactivity

Validated:

Cited:

Applications

Validated:

Cited:

Label

Antibody Source

View all Siglec-E Antibodies »

Product Specifications

Immunogen

Specificity

Clonality

Host

Isotype

Scientific Data Images for Mouse Siglec-E Antibody

Detection of Mouse Siglec-E in Mouse Splenocytes.