Mouse SIGNR3/CD209d Antibody

R&D Systems, part of Bio-Techne | Catalog # AF7220

R&D Systems, part of Bio-Techne
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AF7220
AF7220-SP
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Key Product Details

Species Reactivity

Validated:

Mouse

Cited:

Mouse

Applications

Validated:

Western Blot

Cited:

Flow Cytometry

Label

Unconjugated

Antibody Source

Polyclonal Sheep IgG

View all SIGNR3/CD209d Antibodies »

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant mouse SIGNR3/CD209d
Glu82-Lys237
Accession # Q91ZW8

Specificity

Detects mouse SIGNR3/CD209d in direct ELISAs and Western blots. In direct ELISAs, less than 1% cross-reactivity with recombinant human (rh) DC-SIGN R, rhDC-SIGN, recombinant mouse (rm) SIGN R1, rmSIGN R4, and rmSIGN R7 is observed.

Clonality

Polyclonal

Host

Sheep

Isotype

IgG

Scientific Data Images for Mouse SIGNR3/CD209d Antibody

Detection of Mouse SIGNR3/CD209d antibody by Western Blot.

Detection of Mouse SIGNR3/CD209d by Western Blot.

Western blot shows lysates of J774A.1 mouse reticulum cell sarcoma macrophage cell line and RAW 264.7 mouse monocyte/macrophage cell line. PVDF membrane was probed with 2 µg/mL of Sheep Anti-Mouse SIGNR3/CD209d Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7220) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for SIGNR3/CD209d at approximately 30 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Applications for Mouse SIGNR3/CD209d Antibody

Application
Recommended Usage

Western Blot

2 µg/mL
Sample: J774A.1 mouse reticulum cell sarcoma macrophage cell line and RAW 264.7 mouse monocyte/macrophage cell line
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Sterile PBS to a final concentration of 0.2 mg/mL. For liquid material, refer to CoA for concentration.

Reconstitution Buffer Available:
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Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: SIGNR3/CD209d

SIGNR3 (Specific Intercellular adhesion molecule-3-Grabbing Non-integrin Related gene 3; also CD209d and DC-SIGNR3) is a 30-34 kDa member of the C-type lectin family of proteins. It is found on macrophages, DC and monocytes, and appears to bind both high mannose and fucose moieties on the surface of extracellular pathogens. It is particularly important in clearing Mycobacterium tuberculosis. Upon ligation, SIGNR3 is internalized and presumably enters the endosomal system. Notably, and unlike in human, there are multiple SIGNs in mouse, and SIGNR3 most closely resembles the biology of human DC-SIGN. Mouse SIGNR3 is a 237 amino acid (aa) type II transmembrane glycoprotein. It contains a 54 aa N-terminal cytoplasmic region plus a 162 aa C-terminal extracellular domain (aa 76-237) that possesses a C-type lectin domain (aa 106-228). It is suggested that mouse SIGNR3 may oligomerize. There are two potential splice variants. One possesses an alternative start site 23 aa upstream of the standard site, while a second shows a deletion of aa 45-74. Over aa 82-237, mouse SIGNR3 shares 89% and 59% aa sequence identity with rat and human SIGNR3, respectively.

Long Name

DC-SIGN-related Protein 3

Alternate Names

CD209d

Entrez Gene IDs

170779 (Mouse); 363856 (Rat)

Gene Symbol

CD209D

UniProt

Q91ZW8 (Mouse)

Additional SIGNR3/CD209d Products

Product Documents for Mouse SIGNR3/CD209d Antibody

Product Datasheets

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Mouse SIGNR3/CD209d Antibody

For research use only

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