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Mouse SLPI Antibody

R&D Systems, part of Bio-Techne | Catalog # AF1735

R&D Systems, part of Bio-Techne
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AF1735
AF1735-SP

Key Product Details

Species Reactivity

Validated:

Mouse

Cited:

Mouse

Applications

Validated:

Western Blot

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived mouse SLPI

Specificity

Detects mouse SLPI in direct ELISAs and Western blots. In these formats, less than 5% cross-reactivity with recombinant human SLPI is observed.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Mouse SLPI Antibody

Detection of Mouse SLPI by Western Blot.

Western Blot shows lysates of mouse ovary. PVDF membrane was probed with 2 µg/ml of Goat Anti-Mouse SLPI Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1735) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for SLPI at approximately 12 kDa (as indicated). This experiment was conducted under reducing conditions and using Western Blot Buffer Group 1.

Applications for Mouse SLPI Antibody

Application
Recommended Usage

Western Blot

2 µg/mL
Sample: Mouse ovary

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Reconstitution Buffer Available:
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Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. See Certificate of Analysis for details.
*Small pack size (-SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: SLPI

SLPI is a member of the Serpin family and is primarily expressed in secretory glandular epithelial cells. It is found in various secretory fluids and functions as a potent inhibitor of many proteolytic enzymes. SLPI can modulate cell proliferation and has been shown to have anti-inflammatory, anti-viral, and anti-bacterial activities (1, 2).

References

  1. Zhang, D. et al. (2002) J. Biol. Chem. 277:29999.
  2. Taggart, C.C. et al. (2002) J. Biol. Chem. 277:33648.

Long Name

Secretory Leukocyte Protease Inhibitor

Alternate Names

ALK1, ALPProtease inhibitor WAP4, antileukoproteinase, BLPISeminal proteinase inhibitor, HUSI, HUSI-I, Mucus proteinase inhibitor, secretory leukocyte peptidase inhibitor, Secretory leukocyte protease inhibitor, secretory leukocyte protease inhibitor (antileukoproteinase), WAP four-disulfide core domain protein 4, WAP4HUSI-1, WFDC4MPI

Entrez Gene IDs

6590 (Human); 20568 (Mouse)

Gene Symbol

SLPI

Additional SLPI Products

Product Documents for Mouse SLPI Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Mouse SLPI Antibody

For research use only

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