Mouse Syndecan-2/CD362 Alexa Fluor® 647-conjugated Antibody

R&D Systems, part of Bio-Techne | Catalog # FAB6585R

R&D Systems, part of Bio-Techne
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FAB6585R-100UG
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AF6585
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Key Product Details

Species Reactivity

Mouse

Applications

Flow Cytometry

Label

Alexa Fluor 647 (Excitation = 650 nm, Emission = 668 nm)

Antibody Source

Polyclonal Sheep IgG

View all Syndecan-2/CD362 Antibodies »

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant mouse Syndecan-2/CD362
Accession # P43407

Specificity

Detects mouse Syndecan-2/CD362 in direct ELISAs and Western blots. In direct ELISAs, less than 1% cross-reactivity with recombinant human Syndecan-2/CD362 is observed.

Clonality

Polyclonal

Host

Sheep

Isotype

IgG

Scientific Data Images for Mouse Syndecan-2/CD362 Alexa Fluor® 647-conjugated Antibody

Detection of Syndecan-2/CD362 antibody in NIH-3T3 Mouse Cell Line antibody by Flow Cytometry.

Detection of Syndecan-2/CD362 in NIH-3T3 Mouse Cell Line by Flow Cytometry.

NIH-3T3 mouse cell line was stained with Sheep Anti-Mouse Syndecan-2/CD362 Alexa Fluor® 647-conjugated Antigen Affinity-purified Polyclonal Antibody (Catalog # FAB6585R, filled histogram) or control antibody (Catalog # IC016R, open histogram). View our protocol for Staining Membrane-associated Proteins.

Applications for Mouse Syndecan-2/CD362 Alexa Fluor® 647-conjugated Antibody

Application
Recommended Usage

Flow Cytometry

0.25-1 µg/106 cells
Sample: NIH-3T3 Mouse Cell Line
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

Supplied in a saline solution containing BSA and Sodium Azide.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: Syndecan-2/CD362

Syndecan-2, previously known as fibroglycan or heparan sulfate proteoglycan, is a member of the syndecan family of type 1 transmembrane proteins capable of carrying heparan sulfate (HS) and chondroitin sulfate glycosaminoglycans. The four vertebrate syndecans show conserved cytoplasmic domains and divergent extracellular portions (except for GAG attachment sites). Among the Syndecans, Syndecan-2 is most similar to Syndecan-4 (1‑3). Mouse Syndecan-2 is synthesized as a 202 amino acid (aa) core protein with an 18 aa signal sequence, a 127 aa extracellular domain (ECD), a 25 aa transmembrane region and a 32 aa cytoplasmic tail (4). The ECD of mouse Syndecan-2 contains three closely-spaced consensus Ser-Gly sequences for the attachment of HS side chains. It shares 76%, 86%, 74% and 72% aa identity with the ECD of human, rat, porcine and bovincoe Syndecan-2, respectively. The cytoplasmic tail has both serine and tyrosine phosphorylation sites. Addition of 20 ‑ 80 disaccharides per side chain adds considerably to the size of the 22 kDa core protein. Non-covalent homodimerization of Syndecan-2, or heterodimerization with Syndecan-4, is dependent on the transmembrane domain (5, 6). Syndecan-2 is expressed in cells of mesenchymal origin, neuronal and epithelial cells, and is the predominant syndecan expressed during embryonic development. Expression is up‑regulated in several cancer cell lines (7). After induction in macrophages by inflammatory mediators, Syndecan-2 selectively binds FGF basic, VEGF and EGF (8). Syndecan-2 expressed on human primary osteoblasts binds GM‑CSF and may function as a co-receptor (9). Activated endothelial cell Syndecan-2 specifically binds IL-8 and may participate in promoting neutrophil extravasation by forming a chemotactic IL-8 gradient (10). Typically, cytokine, chemokine and extracellular matrix protein binding occurs through interaction with HS side chains, but the Syndecan-2 extracellular domain can bind TGF-beta directly via protein-protein interaction (11).

References

1. Tkachenko, E. et al. (2005) Circ. Res. 96:488.2. Oh, E.-S, and J. R. Couchman (2004) Mol. Cells 17:181. 3. Essner, J. J. et al. (2006) Int. J. Biochem. Cell Biol. 38:152.4. Marynen, P. et al. (1989) J. Biol. Chem. 264:7017.5. Choi, S. et al. (2005) J. Biol. Chem. 280:42573. 6. Dews, I.C. and K.R. MacKenzie (2007) Proc. Natl. Acad. Sci. USA 104:20782.7. Park, H. et al. (2002) J. Biol. Chem. 277:29730.8. Clasper, S. et al. (1999) J. Biol. Chem. 274:24113.9. Modrowski, D. et al. (2000) J. Biol. Chem. 275:9178.10. Halden, Y. et al. (2004) Biochem. J. 377:533.11. Chen, L. et al. (2004) J. Biol. Chem. 279:15715.

Alternate Names

CD362, Fibroglycan, HSPG1, SDC2, SYND2, Syndecan2

Entrez Gene IDs

6383 (Human); 15529 (Mouse)

Gene Symbol

SDC2

UniProt

P43407 (Mouse)

Additional Syndecan-2/CD362 Products

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Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Mouse Syndecan-2/CD362 Alexa Fluor® 647-conjugated Antibody


This product is provided under an agreement between Life Technologies Corporation and R&D Systems, Inc, and the manufacture, use, sale or import of this product is subject to one or more US patents and corresponding non-US equivalents, owned by Life Technologies Corporation and its affiliates. The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The sale of this product is expressly conditioned on the buyer not using the product or its components (1) in manufacturing; (2) to provide a service, information, or data to an unaffiliated third party for payment; (3) for therapeutic, diagnostic or prophylactic purposes; (4) to resell, sell, or otherwise transfer this product or its components to any third party, or for any other commercial purpose. Life Technologies Corporation will not assert a claim against the buyer of the infringement of the above patents based on the manufacture, use or sale of a commercial product developed in research by the buyer in which this product or its components was employed, provided that neither this product nor any of its components was used in the manufacture of such product. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, Cell Analysis Business Unit, Business Development, 29851 Willow Creek Road, Eugene, OR 97402, Tel: (541) 465-8300. Fax: (541) 335-0354.

For research use only

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