Species Reactivity:

Mouse

Immunogen:

Synthetic Peptide
Accession # Q9JKD8

Applications:

Immunohistochemistry, Western Blot

Label:

Unconjugated

Source:

Monoclonal Rat IgG_2B Clone # 1087415

Specificity:

Detects a synthetic peptide specific for mouse T-bet around amino acid 20 in Direct ELISA.

View Full Technical Details for Mouse T-bet/TBX21 Antibody

Technical Data
Citations / Reviews

Applications for Mouse T-bet/TBX21 Antibody

	Recommended Concentration	Sample
Western Blot	2 µg/mL	HT-2 mouse T cell line
Immunohistochemistry	3-25 µg/mL	Immersion fixed paraffin-embedded sections of mouse spleen

Please Note: Optimal dilutions should be determined by each laboratory for each application.

Scientific Data Examples for Mouse T-bet/TBX21 Antibody

	Immunohistochemistry-Paraffin Detection of T‑bet/TBX21 in Mouse Spleen. T‑bet/TBX21 was detected in immersion fixed paraffin-embedded sections of mouse spleen using Rat Anti-Mouse T‑bet/TBX21 Monoclonal Antibody (Catalog # MAB11627) at 5 µg/ml overnight at 4 °C. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using VisUCyte Antigen Retrieval Reagent-Basic (Catalog # VCTS021). Tissue was stained using the HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005) and counterstained with hematoxylin (blue). Specific staining was localized to the nucleus. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
	Western Blot Detection of Mouse T‑bet/TBX21 by Western Blot. Western Blot shows lysates of HT‑2 mouse T cell line. PVDF membrane was probed with 2 µg/ml of Rat Anti-Mouse T‑bet/TBX21 Monoclonal Antibody (Catalog # MAB11627) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005). A specific band was detected for T‑bet/TBX21 at approximately 60 kDa (as indicated). This experiment was conducted under reducing conditions and using Western Blot Buffer Group 1.

Formulation, Preparation and Storage

Purification	Protein A or G purified from cell culture supernatant
Reconstitution	Reconstitute lyophilized material at 0.2 mg/ml in sterile PBS. For liquid material, refer to CoA for concentration. Reconstitution Buffer Available: Size / Price Qty Loading... RB01:
Formulation	Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Shipping	Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: T-bet/TBX21

T-box transcription factor TBX21, also known as T-bet, is a 58kDa T-box gene which encodes transcription factors involved in the regulation of developmental processes. TBX21 is a Th1 cell-specific transcription that controls the expression of interferon-gamma. TBX21 also has an important role in many cells of the adaptive and innate immune system and is also expressed in dendritic cells, natural killer cells, natural killer T cells and innate lymphoid cells. T-bet contributes to the maintenance of mucosal homeostasis and mucosal immune response. Mice lacking adaptive immune cells and T-bet developed disease similar to human ulcerative colitis.

Item	Value
References	Lazarevic V, Glimcher LH, Lord GM. T-bet: a bridge between innate and adaptive immunity. Nat Rev Immunol. 2013 Nov;13(11):777-89. doi: 10.1038/nri3536. Epub 2013 Oct 11. PMID: 24113868; PMCID: PMC6290922. Mohamed R, Lord GM. T-bet as a key regulator of mucosal immunity. Immunology. 2016 Apr;147(4):367-76. doi: 10.1111/imm.12575. Epub 2016 Feb 23. PMID: 26726991; PMCID: PMC4799884.
Long Name	T-box transcription factor TBX21
Entrez Gene IDs	30009 (Human); 57765 (Mouse); 303496 (Rat)
Alternate Names	T-PET, T-bet, T-box 21, T-box expressed in T cells, T-box protein 21, T-box transcription factor TBX21, TBETT-PET, TBLYM, TBLYMT-cell-specific T-box transcription factor T-bet, TBX21, Tbet, Transcription factor TBLYM

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Mouse T-bet/TBX21 Antibody

R&D Systems, part of Bio-Techne | Catalog # MAB11627

Key Product Details

Applications for Mouse T-bet/TBX21 Antibody

Scientific Data Examples for Mouse T-bet/TBX21 Antibody

Immunohistochemistry-Paraffin

Western Blot

Formulation, Preparation and Storage

Reconstitution Calculator

Background: T-bet/TBX21

Customer Reviews for Mouse T-bet/TBX21 Antibody

Product Documents for Mouse T-bet/TBX21 Antibody

Product Datasheets

COA

Certificate of Analysis

SDS

CONTACT INFORMATION

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