Rat IgG Horseradish Peroxidase-conjugated Antibody Best Seller
R&D Systems, part of Bio-Techne | Catalog # HAF005
Conjugate
Catalog #
Key Product Details
Species Reactivity
Rat
Applications
ELISA, Immunohistochemistry, Simple Western, Western Blot
Label
HRP
Antibody Source
Polyclonal Goat IgG
Product Specifications
Immunogen
Rat IgG
Specificity
Detects rat IgG1, IgG2A, IgG2B heavy and light chains in direct ELISAs and Western blots. In direct ELISAs, less than 1% cross-reactivity with human IgG and rabbit IgG is observed.
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Scientific Data Images for Rat IgG Horseradish Peroxidase-conjugated Antibody
Detection of Rat IgG Primary Antibody by Western Blot.
Western blot shows detection of Rat Anti-Human/Mouse Wnt-5a Monoclonal Antibody (MAB645) using a 1:1000 dilution of HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Detection of Rat IgG Primary Antibody by Simple WesternTM.
Simple Western lane view shows detection of Rat Anti-Mouse S100A9 Monoclonal Antibody ( MAB2065) using a 1:50 dilution of HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.
Detection of Rat IgG Primary Antibody by Immunohistochemistry.
Detection of Vimentin in human kidney using 5 ug/mL of Rat monoclonal anti-human Vimentin primary antibody (MAB2105) followed by 30 min incubation with Goat anti Rat HRP conjugated secondary antibodies (Catalog # HAF005) at 1:20 dilution + DAB chromogen (brown). The tissue was counterstained with Hematoxylin (blue). Control was done by omitting primary antibody.Applications for Rat IgG Horseradish Peroxidase-conjugated Antibody
Application
Recommended Usage
ELISA
Use at a 1:1000 dilution.
Immunohistochemistry
Use at a 1:20
dilution
Simple Western
Use at a 1:50 dilution.
Western Blot
Use at a 1:1000 dilution.
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Reviewed Applications
Read 5 reviews rated 4.4 using HAF005 in the following applications:
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Formulation
Supplied as a 0.2 um filtered solution in Sodium Phosphate and Proclin 300.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Do not freeze.
- 6 months from date of receipt, 2 to 8 °C as supplied.
Background: IgG
R&D Systems offers a range of secondary antibodies and controls for flow cytometry, immunohistochemistry, and Western blotting. We provide species-specific secondary antibodies that are available with a variety of conjugated labels.
Our NorthernLights fluorescent secondary antibodies are bright and resistant to photobleaching. We are currently offering secondary antibodies recognizing mouse, rat, goat, sheep, and rabbit IgG as well as chicken IgY. These reagents are available with three distinct excitation and emission maxima, making them ideal for multi-color fluorescence microscopy.
Long Name
Immunoglobulin G
Alternate Names
Immunoglobulin G, ImmunoglobulinG
Additional IgG Products
Product Documents for Rat IgG Horseradish Peroxidase-conjugated Antibody
Product Specific Notices for Rat IgG Horseradish Peroxidase-conjugated Antibody
For research use only
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