Mouse splenocytes were stained with Rat Anti-Mouse CD3 APC-conjugated Monoclonal Antibody (Catalog # FAB4841A) and either (A) Hamster Anti-Mouse TCR gamma/d Biotinylated Monoclonal Antibody (Catalog # FAB7297B) or (B) Hamster IgG Biotinylated Control (Catalog # BAM011) followed by Streptavidin-Phycoerythrin (Catalog # F0040). View our protocol for Staining Membrane-associated Proteins.

Applications for Mouse TCR gamma/ delta Biotinylated Antibody

Application

Recommended Usage

Flow Cytometry

10 µL/10⁶ cells
Sample: Mouse splenocytes

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Formulation

Supplied in a saline solution containing BSA and Sodium Azide.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Do not freeze.

12 months from date of receipt, 2 to 8 °C as supplied.

Background: TCR gamma/delta

The gamma delta T-cell receptor (TCR) is a heteromer that includes type I transmembrane CD3 gamma and CD3 delta glycoprotein subunits of the Ig superfamily. T gamma delta cells develop as a minor population in the thymus and migrate mainly to in skin and intestinal epithelial layers. Mouse and rat CD3 gamma and CD3 delta are synthesized as 182 and 173 amino acid (aa) precursors that result in 160 and 152 aa mature proteins with 94 and 84 aa extracellular domains (ECD), respectively. The germline ECD sequences of CD3 gamma and CD3 delta share 71% and 76% aa identity between mouse and rat, respectively, while both proteins and species share 57-62% aa identity with human CD3 gamma and CD3 delta. Mouse intraepithelial lymphocytes from the small intestine, which contain a major population of gamma delta T cells, were used as the immunogen for the GL-3 antibody(1).

References

Goodman, T. and L. Lefrancois (1989) J. Exp. Med. 170:2401569.

Alternate Names

delta polypeptide, T3D, TiT3

Entrez Gene IDs

915 (Human); 12500 (Mouse); 25710 (Rat)

Gene Symbol

CD3D

Additional TCR gamma/delta Products

Product Documents for Mouse TCR gamma/ delta Biotinylated Antibody

Product Datasheets

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COA

SDS

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Product Specific Notices for Mouse TCR gamma/ delta Biotinylated Antibody

For research use only

Citations
Reviews

Mouse TCR gamma/ delta Biotinylated Antibody

R&D Systems, part of Bio-Techne | Catalog # FAB7297B

Key Product Details

Species Reactivity

Applications

Label

Antibody Source

View all TCR gamma/delta Antibodies »

Product Specifications

Immunogen

Specificity

Clonality

Host

Isotype

Scientific Data Images for Mouse TCR gamma/ delta Biotinylated Antibody

Detection of TCR gamma/ delta in Mouse Splenocytes by Flow Cytometry.