Mouse TER-119 Biotinylated Antibody
R&D Systems, part of Bio-Techne | Catalog # BAM1125
Conjugate
Catalog #
Key Product Details
Species Reactivity
Validated:
Mouse
Cited:
Mouse
Applications
Validated:
Flow Cytometry, Immunohistochemistry, Immunoprecipitation, Western Blot
Cited:
Immunoprecipitation
Label
Biotin
Antibody Source
Monoclonal Rat IgG2B Clone # TER-119
Product Specifications
Immunogen
C57BL/6 mouse day-14 fetal liver cells
Specificity
Detects mouse TER‑119 in Western blots. This antibody has been shown to react with cells of the erythroid lineage in embryonic yolk sac, fetal liver, adult bone marrow, adult peripheral blood, and adult lymphoid organs (Ikuta, K. et al. (1990) Cell 62:863). TER-119 Antigen is a molecule associated with cell-surface glycophorin A (Kina, T. et al. (2000) Br. J. Haematol. 109:280). The antibody has been shown to react with erythroid cells at differentiation stages from early proerythroblast to mature erythrocyte (Kina, T. et al. (2000) Br. J. Haematol. 109:280).
Clonality
Monoclonal
Host
Rat
Isotype
IgG2B
Scientific Data Images for Mouse TER-119 Biotinylated Antibody
TER‑119 in Mouse Embryo.
TER-119 was detected in immersion fixed frozen sections of mouse embryo (E15) using Rat Anti-Mouse TER-119 Biotinylated Monoclonal Antibody (Catalog # BAM1125) at 25 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Rat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS017) and counter-stained with hematoxylin (blue). Specific staining was localized to red blood cell plasma membranes. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.Applications for Mouse TER-119 Biotinylated Antibody
Application
Recommended Usage
Flow Cytometry
0.25 µg/106 cells
Sample: Mouse bone marrow cells
Sample: Mouse bone marrow cells
Immunohistochemistry
8-25 µg/mL
Sample: Immersion fixed frozen sections of mouse embryo (E15)
Sample: Immersion fixed frozen sections of mouse embryo (E15)
Immunoprecipitation
Kina, T. et al. (2000) Br. J. Haematol. 109:280
Western Blot
Kina, T. et al. (2000) Br. J. Haematol. 109:280
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: TER-119
Additional TER-119 Products
Product Documents for Mouse TER-119 Biotinylated Antibody
Product Specific Notices for Mouse TER-119 Biotinylated Antibody
For research use only
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