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Mouse TLR1 PE-conjugated Antibody

R&D Systems, part of Bio-Techne | Catalog # FAB1475P

R&D Systems, part of Bio-Techne
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FAB1475P

Key Product Details

Species Reactivity

Mouse

Applications

Flow Cytometry

Label

Phycoerythrin (Excitation = 488 nm, Emission = 565-605 nm)

Antibody Source

Polyclonal Goat IgG

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant mouse TLR1
Ser25-Asp581
Accession # Q9EPQ1

Specificity

Detects mouse TLR1 in direct ELISAs and Western blots. In direct ELISAs and Western blots, approximately 30% cross-reactivity with recombinant mouse (rm) TLR6 is observed, 5% cross-reactivity with recombinant human (rh) TLR1 and rmTLR2 is observed, and less than 2% cross-reactivity with rhTLR3 and rhTLR4 is observed.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Mouse TLR1 PE-conjugated Antibody

Detection of TLR1 antibody in A20 Mouse Cell Line antibody by Flow Cytometry.

Detection of TLR1 in A20 Mouse Cell Line by Flow Cytometry.

A20 mouse B cell lymphoma cell line was stained with Goat Anti-Mouse TLR1 PE-conjugated Antigen Affinity-purified Polyclonal Antibody (Catalog # FAB1475P, filled histogram) or isotype control antibody (Catalog # IC108P, open histogram). View our protocol for Staining Membrane-associated Proteins.

Applications for Mouse TLR1 PE-conjugated Antibody

Application
Recommended Usage

Flow Cytometry

10 µL/106 cells
Sample: A20 mouse B cell lymphoma cell line

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

Supplied in a saline solution containing BSA and Sodium Azide.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store the unopened product at 2 - 8° C. Do not use past expiration date. Protect from light.

Background: TLR1

The Toll-like family of molecules are type I transmembrane proteins that serve as pattern recognition receptors for microbial pathogens. There are at least twelve mouse and ten human TLRs that activate the innate immune system following exposure to a variety of microbial species (1, 2). TLRs contain a large number of leucine-rich repeats (LRRs) and a cytoplasmic tail with one Toll/IL-1 receptor (TIR) domain. Mature mouse TLR1 consists of a 557 amino acid (aa) extracellular domain (ECD) with 20 LRRs, a 21 aa transmembrane segment, and a 192 aa cytoplasmic domain (3, 4). Within the ECD, mouse TLR1 shares 60% aa sequence identity with mouse TLR6 and 19%‑27% aa sequence identitity with mouse TLR2, -3, -4, -5, -7, -8, -9, -11, -12, and -13. It shares 73% and 86% aa sequence identity with human and rat TLR1, respectively. TLR1 is expressed on the surface of macrophages, dendritic cells, and tonsillar epithelial cells in ligand-independent association with TLR2 (5‑8). TLR2 additionally associates with TLR6 to form a functional complex with specificity for distinct but related microbial ligands (9‑11). TLR1 and TLR2 cooperate in the recognition of bacterial and protozoal triacylated lipopeptides and glycosylphosphatidylinositols (6, 10‑12). Ligand binding induces TLR1 localization to lipid rafts followed by receptor internalization and activation of NF kappaB (7, 11, 13).

References

  1. Miyake, K. (2007) Semin. Immunol. 19:3.
  2. Hopkins, P.A. and S. Sriskandan (2005) Clin. Exp. Immunol. 140:395.
  3. SwissProt # Q9EPQ1.
  4. Matsushima, N. et al. (2007) BMC Genomics 8:124.
  5. Ochoa, M.-T. et al. (2003) Immunology 108:10.
  6. Takeuchi, O. et al. (2002) J. Immunol. 169:10.
  7. Triantafilou, M. et al. (2006) J. Biol. Chem. 281:31002.
  8. Sandor, F. et al. (2003) J. Cell Biol. 162:1099.
  9. Nakao, Y. et al. (2005) J. Immunol. 174:1566.
  10. Ozinsky, A. et al. (2000) Proc. Natl. Acad. Sci. 97:13766.
  11. Lee, J.Y. et al. (2004) J. Biol. Chem. 279:16971.
  12. Krishnegowda, G. et al. (2005) J. Biol. Chem. 280:8606.
  13. Nishiya, T. and A.L. DeFranco (2004) J. Biol. Chem. 279:19008.
 

Long Name

Toll-like Receptor 1

Alternate Names

CD281

Entrez Gene IDs

7096 (Human); 21897 (Mouse); 305354 (Rat)

Gene Symbol

TLR1

UniProt

Additional TLR1 Products

Product Documents for Mouse TLR1 PE-conjugated Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Mouse TLR1 PE-conjugated Antibody

For research use only

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