Mouse TMEFF1/Tomoregulin-1 Antibody
R&D Systems, part of Bio-Techne | Catalog # AF1995
Key Product Details
Species Reactivity
Mouse
Applications
Immunohistochemistry, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Goat IgG
Product Specifications
Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse TMEFF1/Tomoregulin‑1
Ser37-Thr320
Accession # Q6PFE7
Ser37-Thr320
Accession # Q6PFE7
Specificity
Detects mouse TMEFF1/Tomoregulin‑1 in direct ELISAs and Western blots. In direct ELISAs and Western blots, approximately 40% cross‑reactivity with recombinant human (rh) TMEFF1 is observed and less than 5% cross-reactivity with rhTMEFF2 is observed.
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Scientific Data Images for Mouse TMEFF1/Tomoregulin-1 Antibody
TMEFF1/Tomoregulin-1 in Mouse Spinal Cord.
TMEFF1/Tomoregulin-1 was detected in perfusion fixed frozen sections of mouse spinal cord using 15 µg/mL Goat Anti-Mouse TMEFF1/Tomoregulin-1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1995) overnight at 4 °C. Tissue was stained with the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.Applications for Mouse TMEFF1/Tomoregulin-1 Antibody
Application
Recommended Usage
Immunohistochemistry
5-15 µg/mL
Sample: Perfusion fixed frozen sections of mouse spinal cord
Sample: Perfusion fixed frozen sections of mouse spinal cord
Western Blot
0.1 µg/mL
Sample: Recombinant Mouse TMEFF1/Tomoregulin-1
Sample: Recombinant Mouse TMEFF1/Tomoregulin-1
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: TMEFF1/Tomoregulin-1
TMEFF1, also known as tomoregulin 1 is a type I transmembrane glycoprotein that contains two follistatin modules and an EGF domain in its extracellular domain, a transmembrane domain and a short cytoplasmic tail. The extracellular domain can be released as a soluble protein. TMEFF1 selectively regulates nodal but not activin signaling through direct binding to the nodal co-receptor, Cripto.
Long Name
Transmembrane Protein with EFG-like and two Follistatin-like Domains
Alternate Names
C9orf2, Tomoregulin-1, Tomoregulin1
Gene Symbol
TMEFF1
UniProt
Additional TMEFF1/Tomoregulin-1 Products
Product Documents for Mouse TMEFF1/Tomoregulin-1 Antibody
Product Specific Notices for Mouse TMEFF1/Tomoregulin-1 Antibody
For research use only
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