Mouse TREML2/TLT-2 Antibody
R&D Systems, part of Bio-Techne | Catalog # AF6354
Key Product Details
Species Reactivity
Mouse
Applications
CyTOF-ready, Flow Cytometry, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Sheep IgG
Product Specifications
Immunogen
Chinese hamster ovary cell line CHO-derived recombinant mouse TREML2/TLT-2
His25-Ala270
Accession # Q2LA85
His25-Ala270
Accession # Q2LA85
Specificity
Detects mouse TREML2/TLT-2 in direct ELISAs and Western blots. In direct ELISAs, less than 1% cross-reactivity with recombinant mouse (rm) TREML1, rmTREML4, rmTREM1, rmTREM2b, rmTREM3, rmTREM4, and recombinant human TREM2 is observed.
Clonality
Polyclonal
Host
Sheep
Isotype
IgG
Scientific Data Images for Mouse TREML2/TLT-2 Antibody
Detection of Mouse TREML2/TLT‑2 by Western Blot.
Western blot shows lysates of mouse spleen tissue. PVDF Membrane was probed with 1 µg/mL of Mouse TREML2/TLT-2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6354) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A band was detected for TREML2/TLT-2 at approximately 45 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.
Detection of TREML2/TLT-2 in M1 Mouse Cell Line by Flow Cytometry.
M1 mouse myeloid leukemia cell line was stained with Mouse TREML2/TLT-2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6354, filled histogram) or isotype control antibody (Catalog # 5-001-A, open histogram), followed by NorthernLights™ 557-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # NL010).Applications for Mouse TREML2/TLT-2 Antibody
Application
Recommended Usage
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
Flow Cytometry
2.5 µg/106 cells
Sample: M1 mouse myeloid leukemia cell line
Sample: M1 mouse myeloid leukemia cell line
Western Blot
1 µg/mL
Sample: Mouse spleen tissue
Sample: Mouse spleen tissue
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Sterile PBS to a final concentration of 0.2 mg/mL. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: TREML2/TLT-2
Mouse Trem-like transcript 2 protein (TLT-2; also TREML2) is a 34 kDa (predicted for unglycosylated) type I transmembrane cell surface receptor and member of the Trem family of receptor proteins. Mouse TLT-2 is synthesized as a 329 amino acid (aa) precursor that contains a 24 aa signal sequence, a 246 aa extracellular domain (ECD), a 21 aa transmembrane segment, and a 38 aa cytoplasmic tail. Within the ECD are an Ig-like V-type domain (aa 25-126) and one potential site for N-linked glycosylation. Mature mouse TLT-2 shares 49% aa sequence identity with mature human TLT-2. Cell surface expression of TLT-2 is seen for most of the B and myeloid cell lines. TLT-2 is also expressed constitutively on CD8+ T cells.
Long Name
Triggering Receptor Expressed on Myeloid Cells-like 2
Alternate Names
TLT2
Gene Symbol
TREML2
UniProt
Additional TREML2/TLT-2 Products
Product Documents for Mouse TREML2/TLT-2 Antibody
Product Specific Notices for Mouse TREML2/TLT-2 Antibody
For research use only
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