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Mouse TRIM5 Antibody

R&D Systems, part of Bio-Techne | Catalog # AF3550

R&D Systems, part of Bio-Techne
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AF3550
AF3550-SP

Key Product Details

Species Reactivity

Mouse

Applications

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG

Product Specifications

Immunogen

E. coli-derived recombinant mouse TRIM5
Ala2-Gln249
Accession # AAH38937

Specificity

Detects mouse TRIM5 in direct ELISAs and Western blots. In direct ELISAs, less than 1% cross-reactivity with recombinant human TRIM5 is observed.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Applications for Mouse TRIM5 Antibody

Application
Recommended Usage

Western Blot

0.1 µg/mL
Sample: Recombinant Mouse TRIM5
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

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Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: TRIM5

TRIM5 belongs to the large family of TRIM proteins, which are also named RBCC proteins. These proteins contain a RING domain, one or two B-boxes (CHC3H2 zinc finger motifs), and the coiled-coil domain. Different TRIM proteins contain C-terminals that vary in length and composition. They also have different functions and cellular localization. TRIM5 possesses a B30.2 (also known as RFP-like or PRY-SPRY) C-terminal domain and exists as homotrimers. TRIM5 is a single protein RING finger E3 ubiquitin ligase and has been shown to have antiretroviral activities. Mouse TRIM5 shares 49% and 81% amino acid sequence homology with human and rat TRIM5, respectively.

Long Name

Tripartite Motif-containing 5

Alternate Names

RNF88

Entrez Gene IDs

85363 (Human)

Gene Symbol

TRIM5

UniProt

Additional TRIM5 Products

Product Documents for Mouse TRIM5 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Mouse TRIM5 Antibody

For research use only

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