Mouse Trypsin 3/PRSS3 Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB35651
Key Product Details
Species Reactivity
Mouse
Applications
Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Rat IgG2B Clone # 419910
Product Specifications
Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse Trypsin 3/PRSS3
Phe16-Asn246
Accession # NP_035775
Phe16-Asn246
Accession # NP_035775
Specificity
Detects mouse Trypsin 3/PRSS3 in direct ELISAs and Western blots. In direct ELISAs and Western blots, weak cross-reactivity with recombinant human (rh) Trypsin 3 is observed and no cross-reactivity with rhTrypsin 1 or rhTrypsin 2 is observed.
Clonality
Monoclonal
Host
Rat
Isotype
IgG2B
Applications for Mouse Trypsin 3/PRSS3 Antibody
Application
Recommended Usage
Western Blot
1 µg/mL
Sample: Recombinant Mouse Trypsin 3/PRSS3 (Catalog # 3565-SE)
Sample: Recombinant Mouse Trypsin 3/PRSS3 (Catalog # 3565-SE)
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Trypsin 3/PRSS3
Trypsin 3, encoded by the PRSS3 gene and also known as mesotrypsin is a serine protease and is one of three pancreatic Trypsins. It constitutes less than 10% of the total Trypsin content in normal pancreatic juice. Trypsin 3 is activated by enterokinase cleavage of the 8 aa pro region and secreted into the duodenum lumen. Trypsin 3 is highly resistance to polypeptide Trypsin inhibitors, and may degrade them in foods to facilitate digestion. The 223 amino acid mature mouse Trypsin 3 shares 78% and 96% aa identity with human and rat Trypsin 3, respectively.
Alternate Names
Mesotrypsin, MTG, PRSS3, PRSS4, Trypsin 4, Trypsinogen 15
Gene Symbol
PRSS3
UniProt
Additional Trypsin 3/PRSS3 Products
Product Documents for Mouse Trypsin 3/PRSS3 Antibody
Product Specific Notices for Mouse Trypsin 3/PRSS3 Antibody
For research use only
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