Mouse Trypsin 3/PRSS3 Antibody
R&D Systems, part of Bio-Techne | Catalog # AF3565
Key Product Details
Species Reactivity
Validated:
Mouse
Cited:
Mouse
Applications
Validated:
Immunohistochemistry, Immunoprecipitation, Western Blot
Cited:
Immunohistochemistry
Label
Unconjugated
Antibody Source
Polyclonal Goat IgG
Product Specifications
Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse Trypsin 3
Phe16-Asn246
Accession # NP_035775
Phe16-Asn246
Accession # NP_035775
Specificity
Detects mouse Trypsin 3 in direct ELISAs and Western blots. In direct ELISAs, approximately 20% cross-reactivity with recombinant human (rh) Trypsin 1 is observed.
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Scientific Data Images for Mouse Trypsin 3/PRSS3 Antibody
Trypsin 3/PRSS3 in Mouse Pancreas.
Trypsin 3/PRSS3 was detected in perfusion fixed frozen sections of mouse pancreas using 5 µg/mL Goat Anti-Mouse Trypsin 3/PRSS3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3565) overnight at 4 °C. Tissue was stained with the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.
Trypsin 3/PRSS3 in Mouse Pancreas.
Trypsin 3/PRSS3 was detected in perfusion fixed frozen sections of mouse pancreas using Mouse Trypsin 3/PRSS3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3565) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Lower panel shows a lack of labeling if primary antibodies are omitted and tissue is stained only with secondary antibody followed by incubation with detection reagents. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.Applications for Mouse Trypsin 3/PRSS3 Antibody
Application
Recommended Usage
Immunohistochemistry
5-15 µg/mL
Sample: Perfusion fixed frozen sections of mouse pancreas
Sample: Perfusion fixed frozen sections of mouse pancreas
Immunoprecipitation
25 µg/mL
Sample: Conditioned cell culture medium spiked with Recombinant Mouse Trypsin 3/PRSS3 (Catalog # 3565-SE), see our available Western blot detection antibodies
Sample: Conditioned cell culture medium spiked with Recombinant Mouse Trypsin 3/PRSS3 (Catalog # 3565-SE), see our available Western blot detection antibodies
Western Blot
0.1 µg/mL
Sample: Recombinant Mouse Trypsin 3/PRSS3 (Catalog # 3565-SE)
Sample: Recombinant Mouse Trypsin 3/PRSS3 (Catalog # 3565-SE)
Reviewed Applications
Read 1 review rated 5 using AF3565 in the following applications:
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Trypsin 3/PRSS3
Mouse Trypsin 3, encoded by the PRSS3 gene, is also known as mesotrypsin. It consists of a signal peptide (residues 1-15), a pro region (residues 16-23), and a mature chain (residues 24-246) (1). The purified recombinant mouse Trypsin 3 corresponds to the pro form, which can be activated by enterokinase.
References
- Halfon, S. et al. (2004) in Handbook of Proteolytic Enzymes (ed. Barrett, A.J. et al.) pp. 1483, Academic Press, San Diego.
Alternate Names
Mesotrypsin, MTG, PRSS3, PRSS4, Trypsin 4, Trypsinogen 15
Gene Symbol
PRSS3
UniProt
Additional Trypsin 3/PRSS3 Products
Product Documents for Mouse Trypsin 3/PRSS3 Antibody
Product Specific Notices for Mouse Trypsin 3/PRSS3 Antibody
For research use only
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