Mouse TSLP Antibody

Cell Proliferation Induced by TSLP and Neutralization by Mouse TSLP Antibody.

Recombinant Mouse TSLP (Catalog # 555-TS) stimulates proliferation in the BaF3 mouse pro-B cell line transfected with mouse IL-7 Ra in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Mouse TSLP (7.5 ng/mL) is neutralized (green line) by increasing concentrations of Mouse TSLP Monoclonal Antibody (Catalog # MAB555). The ND₅₀ is typically 0.1-0.4 µg/mL.

Detection of Mouse TSLP by Block/Neutralize

TSLP neutralization inhibits airway structural alterations.(A) The depth of peribronchial collagen deposition was significantly reduced in anti-TSLP-treated mice, compared with HDM-exposed and IgG isotype-treated controls. (B) Epithelial goblet cells were markedly reduced in anti-TSLP-treated mice, compared to the HDM-exposed and IgG -treated controls. (C) TGF-beta 1 levels in the BALF were significantly increased in HDM-exposed mice. The blockage of TSLP with the anti-TSLP mAb reduced TGF-beta 1levels in the BALF. TGF-beta 1 protein and mRNA levels were determined in duplicate experiments. (D-G) Representative photomicrographs of Masson's trichrome-stained lung sections from mice that were exposed for 5 weeks to saline (D) or HDM (E) and pretreated with anti-TSLP mAb (F) or an IgG-treated control (G) 60 minutes prior to and during HDM exposure. (H-K) Representative photomicrographs of airway sections of PAS-stained from mice that were exposed for 5 weeks to saline (H) or HDM (I) and pretreated with anti-TSLP mAb (J) or an IgG-treated control (K) 60 minutes prior to and during the HDM exposure. Original magnification, 20×. The data shown represent the means±SEM (n = 5), **p<0.01 compared with the HDM group, ##p<0.01 compared with the IgG-treated control mice. The photomicrographs of H-K are representative of 5 independent experiments. Image collected and cropped by CiteAb from the following publication (https://dx.plos.org/10.1371/journal.pone.0051268), licensed under a CC-BY license. Not internally tested by R&D Systems.

Detection of Mouse Mouse TSLP Antibody by Flow Cytometry

TSLP neutralization inhibits surface marker expression on airway CD11c+ cells.(A) Lung cell suspensions from mice were stained with combinations of PerCP-Cy 5.5-conjugated CD11c, PE-conjugated OX40L, FITC-conjugated CD80 and APC-conjugated CD86. The dead cells and the debris were excluded based on forward scatter (FSC)/side scatter (SSC) plots. (B) A total of 1×105 to 3×105 viable cells were acquired using FACS, and the DCs were then sorted as CD11c+ airway cells on FSC/SSC plots. (C) Mice that were chronically exposed to HDM received an intranasal administration of anti-TSLP mAb or of a control IgG 60 minutes prior to each HDM exposure. The expression levels of OX40L, CD80 and CD86 on pulmonary DCs were then analyzed using flow cytometry. The HDM-exposed mice exhibited a significant increase in the OX40L, CD80 and CD86 surface markers on CD11c+ airway cells. However, anti-TSLP pretreatment effectively reduced OX40L, CD80 and CD86 expression on the DCs, even though the mice were continuously exposed to HDM. (D) The TSLP levels in the BALF were highly correlated with OX40L expression on CD11c+ airway cells in all of the treatment mice (n = 20, R = 0.879, p<0.01). (E) Representative data from one of the five replicates. (F) Staining for OX40L (12-4031), CD80 (11-4888), and CD86 (17-4321) from isotype-Ig-treated controls. The data represent the means±SEM (n = 5). * p<0.05 or ** p<0.01 compared with the HDM group. # p<0.05 or ## p<0.01 compared with the IgG-treated control mice. The results are derived from 4 experimental groups (5 mice/per group), and the data are representative of 5 independent experiment. Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/23300949), licensed under a CC-BY license. Not internally tested by R&D Systems.