CMT-93 mouse rectal carcinoma cell line was stained with Rat Anti-Mouse TSPAN8 PE-conjugated Monoclonal Antibody (Catalog # FAB6524P, filled histogram) or isotype control antibody (Catalog # IC013P, open histogram). View our protocol for Staining Membrane-associated Proteins.

Applications for Mouse TSPAN8 PE-conjugated Antibody

Application

Recommended Usage

Flow Cytometry

10 µL/10⁶ cells
Sample: CMT‑93 mouse rectal carcinoma cell line

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Formulation

Supplied in a saline solution containing BSA and Sodium Azide.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store the unopened product at 2 - 8° C. Do not use past expiration date. Protect from light.

Background: TSPAN8

TSPAN8 (Tetraspanin-8), also known as TM4SF3 and CO-029, is a 27-33 kDa member of the tetraspanin family of proteins. As evidenced by its name, it is a 4-transmembrane (glyco)protein that is found on/in multiple tumor types as well as smooth and skeletal muscle, endothelium and hematopoietic progenitor cells. All 4-transmembrane proteins are not tetraspanins. Tetraspanins are characterized by the presence of a very short intracellular C- and N-terminus, and a very long extracellular (or LEL) domain linking the 3rd and 4th transmembrane segments. On TSPAN8 this domain is 98 amino acids (aa) in length and represents 42% of the entire length of mouse TSPAN8 (or 235 aa). TSPAN8, like other tetraspanins, forms the backbone of a network that allows molecule interactions on (or in) plasma membranes. This ability is dependent upon molecule palmitoylation and creates a microdomain (or TEM) that is distinct from typical membrane rafts, the latter of which is characterized by the presence of GPI-linked proteins and the molecule caveolin. Within this TEM, TSPAN8 may form homodimers, and is known to interact with multiple molecule types, including CD151, CD81, CD9, CD13, EpCAM, EWI-F, MMP-9, Meprin-beta, Rictor, TACE and the integrins alpha3(CD49c) beta1, alpha6 beta4 and alpha4(CD49d) beta1. Such interactions appear to positively or negatively modulate individual molecule activity. These interactions are generally described within the context of exosomes, 30-100 nm membrane-bound vesicles that are released from multiple cell types. TSPAN8-containing exosomes are reported to either facilitate cell motility or act as an antiapoptotic agent for target cells. Over aa 106-203 constituting the LEL, mouse TSPAN8 shares 58% and 73% aa sequence identity with human and rat TSPAN8, respectively.

Long Name

Tetraspanin 8/Transmembrane 4 Superfamily, Member 3

Alternate Names

CO-029, TM4SF3

Entrez Gene IDs

7103 (Human); 216350 (Mouse); 171048 (Rat)

Gene Symbol

TSPAN8

UniProt

Q8R3G9

Additional TSPAN8 Products

Product Documents for Mouse TSPAN8 PE-conjugated Antibody

Product Datasheets

Download Product Datasheets

COA

SDS

Download SDS

Product Specific Notices for Mouse TSPAN8 PE-conjugated Antibody

For research use only

Citations
Reviews

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Flow Cytometry

Mouse TSPAN8 PE-conjugated Antibody

R&D Systems, part of Bio-Techne | Catalog # FAB6524P

Key Product Details

Species Reactivity

Validated:

Cited:

Applications

Validated:

Cited:

Label

Antibody Source

View all TSPAN8 Antibodies »

Product Specifications

Immunogen

Specificity

Clonality

Host

Isotype

Scientific Data Images for Mouse TSPAN8 PE-conjugated Antibody

Detection of TSPAN8 in CMT‑93 Mouse Cell Line by Flow Cytometry.