Detects mouse Tyro3/Dtk extracellular domain in direct ELISAs and Western blots. In direct ELISAs and Western blots, no cross-reactivity with recombinant human Tyro3/Dtk, recombinant mouse (rm) Mer, or rmAxl is observed.

Clonality

Monoclonal

Host

Rat

Isotype

IgG₁

Scientific Data Images for Mouse Tyro3/Dtk Antibody

Detection of Tyro3/Dtk in Transfected HEK 293 (positive) and mIMCD-3 (positive).

Tyro3/Dtk was detected in immersion fixed Transfected HEK293 human embryonic kidney cells (positive) and mIMCD-3 mouse epithelial cell line (positive) using Rat Anti-Mouse Tyro3/Dtk Monoclonal Antibody (Catalog # MAB759) at 25 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Rat IgG Secondary Antibody (red; Catalog # NL013) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Detection of Tyro3/Dtk in Transfected HEK 293 (positive) and wild-type HEK 293 (negative) cells.

Tyro3/Dtk was detected in immersion fixed Transfected HEK293 human embryonic kidney cells (positive) and absent in wild-type HEK293 human embryonic kidney cells (negative) using Rat Anti-Mouse Tyro3/Dtk Monoclonal Antibody (Catalog # MAB759) at 25 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Rat IgG Secondary Antibody (red; Catalog # NL013) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Applications for Mouse Tyro3/Dtk Antibody

Application

Recommended Usage

Immunocytochemistry

5-25 µg/mL
Sample: Immersion fixed Transfected HEK293 human embryonic kidney cells (positive), mIMCD-3 mouse epithelial cells (positive), and wild-type HEK293 human embryonic kidney cells (negative)

Western Blot

1 µg/mL
Sample: Recombinant Mouse Tyro3/Dtk Fc Chimera (Catalog # 759-DT)

Mouse Tyro3/Dtk Sandwich Immunoassay

ELISA Capture (Matched Antibody Pair)

Recommended Concentration: 2-8 µg/mL
Use in combination with these reagents:

Detection Reagent: (Catalog # BAF759)
Standard: Recombinant Mouse Tyro3/Dtk Fc Chimera Protein, CF (Catalog # 759-DT)

Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Tyro3/Dtk

Axl (Ufo, Ark), Dtk (Sky, Tyro3, Rse, Brt) and Mer (human and mouse homologues of chicken c-Eyk) constitute a new receptor tyrosine kinase subfamily. The extracellular domain of these proteins contain two Ig-like motifs and two fibronectin type III motifs. This characteristic topology is also found in neural cell adhesion molecules and in receptor tyrosine phosphatases. All three receptors bind the vitamin K-dependent protein growth-arrest specific gene 6 (Gas6) which is structurally related to the anticoagulation factor protein S. The binding affinities for Gas6 is in the order of Axl > Dtk > Mer. Gas6 binding induces tyrosine phosphorylation and downstream signaling pathways that can lead to cell proliferation, migration, or the prevention of apoptosis. Dtk is widely expressed during embryonic development. In adults, Dtk is predominantly expressed in neurons in restricted regions of the brain.

References

Nagata, K. et al. (1996) J. Biol. Chem. 22:30022.
Crosier, K.E. and P.S Crosier (1997) Pathology 29:131.

Long Name

Tyrosine Protein Kinase Receptor TYRO3

Alternate Names

Brt, Dtk, Rse, Sky

Entrez Gene IDs

7301 (Human); 22174 (Mouse); 102135978 (Cynomolgus Monkey)

Gene Symbol

TYRO3

UniProt

P55144

Additional Tyro3/Dtk Products

Product Documents for Mouse Tyro3/Dtk Antibody

Product Datasheets

Download Product Datasheets

COA

SDS

Download SDS

Product Specific Notices for Mouse Tyro3/Dtk Antibody

For research use only

Citations
Reviews

Mouse Tyro3/Dtk Antibody

R&D Systems, part of Bio-Techne | Catalog # MAB759

Key Product Details

Species Reactivity

Validated:

Cited:

Applications