Mouse ULBP‑1/MULT-1 Alexa Fluor® 700‑conjugated Antibody
R&D Systems, part of Bio-Techne | Catalog # FAB2588N
Conjugate
Catalog #
Key Product Details
Species Reactivity
Mouse
Applications
Flow Cytometry
Label
Alexa Fluor 700 (Excitation = 675-700 nm, Emission = 723 nm)
Antibody Source
Monoclonal Rat IgG2A Clone # 237104
Product Specifications
Immunogen
BaF3 mouse pro-B cell line transfected with mouse ULBP‑1 /MULT-1
Specificity
Detects mouse ULBP‑1 /MULT-1 in flow cytometry.
Clonality
Monoclonal
Host
Rat
Isotype
IgG2A
Scientific Data Images for Mouse ULBP‑1/MULT-1 Alexa Fluor® 700‑conjugated Antibody
Detection of ULBP‑1 in A20 Mouse Cell Line by Flow Cytometry.
A20 mouse B cell lymphoma cell line was stained with Rat Anti-Mouse ULBP-1 Alexa Fluor® 700-conjugated Monoclonal Antibody (Catalog # FAB2588N, filled histogram) or isotype control antibody (Catalog # IC006N, open histogram). View our protocol for Staining Membrane-associated Proteins.Applications for Mouse ULBP‑1/MULT-1 Alexa Fluor® 700‑conjugated Antibody
Application
Recommended Usage
Flow Cytometry
0.25-1 µg/106 cells
Sample: A20 mouse B cell lymphoma cell line
Sample: A20 mouse B cell lymphoma cell line
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Formulation
Supplied 0.2 mg/mL in a saline solution containing BSA and Sodium Azide.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
- 12 months from date of receipt, 2 to 8 °C as supplied.
Background: ULBP-1
ULBP-1, also known as MULT-1 (Mouse UL16-Binding Protein-like Transcript 1), is a 53 kDa, MHC Class I-like molecule that belongs to the mouse family of NKG2D ligands (1‑4). It is a type I transmembrane glycoprotein that is synthesized as a 334 amino acid (aa) precursor. It contains a 25 aa signal sequence, a 186 aa extracellular region, a 19 aa transmembrane segment and a 104 aa cytoplasmic tail (2). The extracellular region contains an alpha1 and alpha2 like domain with two intrachain disulfide bonds. ULBP-1 is distantly related to other human and mouse NKG2D ligands, and more distantly related to the MHC class I proteins (3). Unlike most NKG2D ligands, transcripts for ULBP-1 have been detected in a wide variety of mouse tissues and tumor cells lines (3). The receptor for ULBP-1 is NKG2D, a 35 kDa C-type lectin that is found on mouse NK cells, activated CD8+ T cells, epidermal gamma delta T cells, and activated macrophages (1, 5, 6, 7). Recombinant ULBP-1 protein binds to NKG2D with high affinity (KD = 6 nM) (2). Although an activating receptor, general cellular responses to NKG2D ligation depend upon the isoform of NKG2D and the cell type (5). Exposure to immobilized ULBP-1 or ULBP-1-transfected cells elicits IFN-gamma production by NK cells (3). Ectopic expression of ULBP-1 on the RMA mouse tumor cell line leads to tumor rejection in syngeneic mice (3).
References
- Raulet, D.H. (2003) Nat. Rev. Immunol. 3:781.
- Carayannopoulos, L. et al. (2002) J. Immunol. 169:4079.
- Diefenbach, A. et al. (2003) Eur. J. Immunol. 33:381.
- Krmpotic, A. et al. (2005) J. Exp. Med. 201:211.
- Diefenbach, A. et al. (2002) Nat. Immunol. 3:1142.
- Ho, E.L. et al. (1998) Proc. Natl. Acad. Sci. USA 95:6320.
- Carayannopoulos, L.N. et al. (2002) Eur. J. Immunol. 32:597.
Long Name
UL16 Binding Protein-1
Alternate Names
ALCAN-beta, MULT-1, NKG2DL, NKG2DL1, RAET1I, ULBP1
Gene Symbol
ULBP1
Additional ULBP-1 Products
Product Specific Notices for Mouse ULBP‑1/MULT-1 Alexa Fluor® 700‑conjugated Antibody
This product is provided under an agreement between Life Technologies Corporation and R&D Systems, Inc, and the manufacture, use, sale or import of this product is subject to one or more US patents and corresponding non-US equivalents, owned by Life Technologies Corporation and its affiliates. The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The sale of this product is expressly conditioned on the buyer not using the product or its components (1) in manufacturing; (2) to provide a service, information, or data to an unaffiliated third party for payment; (3) for therapeutic, diagnostic or prophylactic purposes; (4) to resell, sell, or otherwise transfer this product or its components to any third party, or for any other commercial purpose. Life Technologies Corporation will not assert a claim against the buyer of the infringement of the above patents based on the manufacture, use or sale of a commercial product developed in research by the buyer in which this product or its components was employed, provided that neither this product nor any of its components was used in the manufacture of such product. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, Cell Analysis Business Unit, Business Development, 29851 Willow Creek Road, Eugene, OR 97402, Tel: (541) 465-8300. Fax: (541) 335-0354.
For research use only
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