bEnd.3 mouse endo-thelioma cell line was stained with Rat Anti-Mouse VEGFR2/KDR/Flk-1 Mono-clonal Antibody (Catalog # MAB4432, filled histogram) or isotype control antibody (Catalog # MAB005, open histogram), followed by Allophycocyanin-conjugated Anti-Rat IgG F(ab')₂Secondary Antibody (Catalog # F0113).

VEGFR2/KDR/Flk‑1 in bEnd.3 Mouse Cell Line.

VEGFR2/KDR/Flk-1 was detected in immersion fixed bEnd.3 mouse endothelioma cell line using 10 µg/mL Rat Anti-Mouse VEGFR2/KDR/Flk-1 Monoclonal Antibody (Catalog # MAB4432) for 3 hours at room temperature. Cells were stained with the NorthernLights™ 557-conjugated Anti-Rat IgG Secondary Antibody (red; Catalog # NL013) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Applications for Mouse VEGFR2/KDR/Flk-1 Antibody

Application

Recommended Usage

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Flow Cytometry

2.5 µg/10⁶ cells
Sample: bEnd.3 mouse endothelioma cell line

Immunocytochemistry

8-25 µg/mL
Sample: Immersion fixed bEND.3 mouse endothelioma cell line

Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: VEGFR2/KDR/Flk-1

VEGFR2 (KDR/Flk-1), VEGFR1 (Flt-1) and VEGFR3 (Flt-4) belong to the class III subfamily of receptor tyrosine kinases (RTKs). All three receptors contain seven immunoglobulin-like repeats in their extracellular domains and kinase insert domains in their intracellular regions. The expression of VEGFR1, 2, and 3 is almost exclusively restricted to endothelial cells. These receptors are likely to play essential roles in vasculogenesis and angiogenesis. Mature mouse VEGFR2 is composed of a 743 amino acid (aa) extracellular domain, a 22 aa transmembrane domain, and a 583 aa cytoplasmic domain. In contrast to VEGFR1 which binds both PlGF and VEGF with high affinity, VEGFR2 binds VEGF but not PlGF with high affinity.

References

Ferra, N. and R. Davis-Smyth (1997) Endocrine Reviews 18:4.
Achen, M.G. et al. (1998) Proc. Natl. Acad. Sci. USA 95:548.

Long Name

Vascular Endothelial Growth Factor Receptor 2

Alternate Names

CD309, Flk-1, Flk1, KDR, KRD1, Ly73, VEGF R2

Entrez Gene IDs

3791 (Human); 16542 (Mouse)

Gene Symbol

KDR

UniProt

P35918

Additional VEGFR2/KDR/Flk-1 Products

Product Documents for Mouse VEGFR2/KDR/Flk-1 Antibody

Product Datasheets

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COA

SDS

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Product Specific Notices for Mouse VEGFR2/KDR/Flk-1 Antibody

For research use only

Citations
Reviews

Calculators
Flow Cytometry

Mouse VEGFR2/KDR/Flk-1 Antibody

R&D Systems, part of Bio-Techne | Catalog # MAB4432

Key Product Details

Species Reactivity

Validated:

Cited:

Applications

Validated:

Cited:

Label

Antibody Source

View all VEGFR2/KDR/Flk-1 Antibodies »

Product Specifications

Immunogen

Specificity

Clonality

Host

Isotype

Scientific Data Images for Mouse VEGFR2/KDR/Flk-1 Antibody

Detection of VEGFR2/KDR/Flk‑1 in bEnd.3 Mouse Cell Line by Flow Cytometry.